Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1988-01-11
1993-04-27
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530402, 530403, 530404, 424 851, 424 852, 424 8591, 930141, 435 695, 435 6952, C07K 1300, A61K 3702
Patent
active
052063445
ABSTRACT:
Muteins of IL-2 in which one of the amino acids of the mature native sequence of IL-2 is replaced by a cysteine residue are prepared and conjugated through the replaced cysteine residue to a polymer selected from polyethylene glycol homopolymers or polyoxyethylated polyols, wherein said homopolymers are unsubstituted or substituted at one end with an alkyl group. These muteins are made via host expression of mutant genes encoding the muteins that have been changed from the genes for the parent proteins by site-directed mutagenesis. In addition, other species of IL-2 may be conjugated via the cysteine residue at position 125 of the mature IL-2 protein that is not necessary for the biological activity of the IL-2.
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Goodson Robert J.
Halenbeck Robert F.
Katre Nandini
Knauf Michael J.
McCabe Peter C.
Cetus Oncology Corporation
Draper Garnette D.
Hasak Janet E.
McGarrigle Philip L.
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