Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-06-21
1996-03-26
Draper, Garnette D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352402, 4352523, 43525411, 4353201, 536 234, 536 235, 536 241, 530351, 424 852, C12N 1524, C12N 1527, C07K 1454, A61K 3820
Patent
active
055019625
ABSTRACT:
The present invention relates to recombinant human interleukin-3 (hIL-3) variant or mutant proteins (muteins) in which segments of the polypeptide sequence of the human IL-3 polypeptide have been replaced by segments of the murine (mouse) interleukin-3 (mIL-3) polypeptide to form human/murine chimeric hybrid polypeptides. The human/mouse hybrid IL-3 may have amino acid deletions at the N-terminus or the C-terminus or at both the N- and C- termini and in some cases may also contain additional amino acid substitutions or deletions. The human/murine IL-3 muteins retain at least one biological activity of native hIL-3 and may also exhibit an improved side effects profile such as a reduction in the stimulation of leukotriene release or histamine release. The invention also relates to pharmaceutical compositions containing the h/m IL-3 hybrids and methods for using them. Additionally, the present invention relates to recombinant expression vectors comprising nucleotide sequences encoding the IL-3 hybrid muteins, related microbial expression systems, and processes for making the IL-3 hybrids using the microbial expression systems.
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Braford-Goldberg Sarah R.
Easton Alan M.
Klein Barbara K.
McKearn John P.
Olins Peter O.
Bennett Dennis A.
Draper Garnette D.
Fitzgerald David L.
G. D. Searle & Co.
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