Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1979-06-04
1981-04-14
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 68, 435172, 435 5, 435240, 435811, 424 85, C12P 1934
Patent
active
042620902
ABSTRACT:
Method for preparing interferon, mRNA for interferon, and competent recombinant DNA containing dsDNA and cDNA from mRNA coding for mammalian interferon. The method employs crossing a mutant mammalian cell which is semiconstitutive for interferon with a cell derived from the same or different mammal having wild type gene(s) for interferon and for the regulation of interferon synthesis and desirably having phenotypic properties allowing for selection of the hybrid cells. The desired hybrid clones are then induced to produce IF mRNA, wherein the amounts of mRNA for interferon are greatly enhanced over the amounts normally obtained from wild type cell strains. The mRNA is employed to produce cDNA which codes for the mammalian interferon. The single stranded cDNA is employed as a template to prepare dsDNA which is then combined with a replicon recognized by a microorganism host to provide a recombinant DNA. The microorganism host is transformed with the recombinant DNA, so as to provide a source for the interferon gene, as well as interferon.
REFERENCES:
patent: 3773924 (1973-11-01), Ho et al.
Jarvis et al., Somatic Cell Genetics, vol. 4, No. 6, pp. 677-697 (1978).
King, Handbook of Genetics, vol. 5, pp. 267-289 (1976).
Colby, Jr. Clarence
Denney, Jr. Dan W.
Cetus Corporation
Rowland Bertram I.
Tanenholtz Alvin E.
LandOfFree
Interferon production does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Interferon production, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Interferon production will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-159596