Interference-resistant composition, device and method for determ

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435188, 435805, 23230B, 23931, 252408, C12Q 128

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active

043106269

ABSTRACT:
A composition, test device and method of use in determining the presence of a peroxidatively active component in a test sample are disclosed. The composition comprises an organic peroxide, an indicator capable of providing a detectable response in the presence of the peroxide and a peroxidatively active substance, and a complex of Co(III). Inclusion of the Co(III) complex renders the composition resistant to the adverse effects of ascorbate, which might be present in the test sample. The test device comprises a carrier matrix incorporated with the composition. The method comprises contacting the test sample with the device and observing a detectable response therein.

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I. M. Reibel, "Decomposition of Hydrogen Peroxide Solutions in the Presence of Trivalent Cobalt Complex Compounds", Khim. Perekismykh Soedin. Akad. Nauk SSS Inst. Okis. i Neorgan Khim., (1963) pp. 68-79.
"The Merck Index", Ninth Edition, Merck & Co. Inc., Rahway, (1976), p. 311.

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