Interative method of at least three cycles for the refolding of

Details Interative method of at least three cycles for the refolding of Interative method of at least three cycles for the refolding of

1995-06-06

1998-04-14

Walsh, Stephen

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530402, 530412, 530427, 530384, 5303873, 435183, C07K 1400, C07K 14745, C07K 1600, C12P 2100

Patent

active

057392811

ABSTRACT:
A novel, generally applicable method for producing correctly folded proteins from a mixture of misfolded proteins, e.g. bacterial inclusion-body aggregates. A major new aspect of the method is that over-all efficiency is achieved by subjecting proteins to a time-sequence of multiple denaturation-renaturation cycles, resulting in gradual accumulation of the correctly folded protein. The method has proven efficient for a variety of recombinant proteins. Also provided are novel encrypted recognition sites for bovine coagulation factor X.sub.a. The encrypted recognition sites described may be activated in vitro by controlled oxidation or by reversible derivatization of cysteine residues and thereby generate new cleavage sites for factor X.sub.a. Two new recombinant serine protease exhibiting narrow substrate specificity for factor X.sub.a recognition sites are also provided. They may replace natural coagulation factor X.sub.a for cleavage of chimeric proteins.

REFERENCES:
patent: 4444878 (1984-04-01), Paulus
patent: 4656255 (1987-04-01), Seely
patent: 4999422 (1991-03-01), Galliher
patent: 5074977 (1991-12-01), Cheung et al.
patent: 5077392 (1991-12-01), Rudolph et al.
patent: 5231168 (1993-07-01), Dziegiel et al.
patent: 5284933 (1994-02-01), Dobeli et al.
patent: 5324436 (1994-06-01), John et al.
Reeck et al., 1987, Homology in Proteins and nucleic acids: a terminology muddle and a way out of it, Cell, 50: 667.
Sleigh, 1993, Antibody-based therapeutics -the third generation, Australasian Biotech., 3: 328-331.
Zhang et al., "Reconstitution of the Diiron Sites in Hemerythrin and Myo-hemerythrin", Biochem. 30: 583-589 (1991).
Katagiri et al, "Bovine endo Thelial cell plasminogen activator inhibitor" Eur. J. Biochem. 176 : 81-87 (1988).
Holliger et al "`Diabodies`: Small bivalent and bispecific antibody fragments" PNAS 90: 6444-6448 (Jul. 1993).
Messier et al, Gene 99 : 291-294 (1991) "Cloning and expression in COS-1 cells of a full-length cDNA encoding human coagulation factor X".
Morita et al, "Localization of the Structural Difference between Bovine Blood Coagulation Factors X.sub.1 and X.sub.2 . . .", J. Biol. Chem. 261(9):4008-4014 (Mar. 1986).
Kaul et al "Isolation and characterization of human factor X cDNA" Gene 41 : 311-314 (1986).
Mozhaev et al, Chem Abst. 91:248, abst#119,502; (1979).
Hoffmann et al, "Purification of uis-tagged proteins . . ." Nuc. Acids Res. 19(22): 6337-6338 (1991).
Brey et al, "Dielectric Measurements of Water Sorbed on Ovalbumin and Lysozyme", J. Cell. Interface Sci. 30(1): 13-20 (May 1969).
Buchner, J., et al., "Renaturation, Purification and Characterization of Recombinant F.sub.ab -Fragments Produced in Escherichia Coli", 1991, Biotechnology, vol. 9, pp. 157-162.
Dalb.o slashed.ge, H., et al., "A Novel Enzymatic Method for Production of Authentic hGH From an Escherichia Coli Produced hGH-Precursor", 1987, Bio/Technology, vol. 5, pp. 161-164.
Fung, M., et al., "Blood Coagulation Factor X mRNA Encodes a Single Polypeptide Chain Containing a Prepro Leader Sequence", 1984, Nucleic Acids Research, vol. 12, pp. 4481-4492.
Jaenicke, R., et al., MRC Lab. of Molec. Biol., Cambridge, UK, "Folding Proteins", 1989, Protein Structure, A Practical Approach, IRL Press, pp. 191-223.
Nagai, K., et al., "Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia Coli", 1987, Methods in Enzymology, vol. 153, pp. 461-481.
Schein, C., "Solubility as a Function of Protein Structure and Solvent Components", 1990, Bio/Technology, vol. 8, pp. 308-317.
Holtet, T.H. et al., Febs Letters, vol. 344, No. 2,3, May 16, 1994, pp. 242-246.

Affiliated with

Also associated with

Rating

Interative method of at least three cycles for the refolding of does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Interative method of at least three cycles for the refolding of , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Interative method of at least three cycles for the refolding of will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-635875