Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1996-08-30
1999-12-07
Achutamurthy, Ponnathapura
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
530350, 5303871, 5303911, 530395, 530399, 530403, 530404, 530405, 530406, 424 7808, 4241781, 4241931, 4242801, 424 941, C07K 1708, A61K 374, A61K 39385, A61K 39395
Patent
active
059985887
ABSTRACT:
The combination of the capabilities of stimuli-responsive components such as polymers and interactive molecules to form site-specific conjugates which are useful in a variety of assays, separations, processing, and other uses is disclosed. The polymer chain conformation and volume can be manipulated through alteration in pH, temperature, light, or other stimuli. The interactive molecules can be biomolecules like proteins or peptides, such as antibodies, receptors, or enzymes, polysaccharides or glycoproteins which specifically bind to ligands, or nucleic acids such as antisense, ribozymes, and aptamers, or ligands for organic or inorganic molecules in the environment or manufacturing processes. The stimuli-responsive polymers are coupled to the recognition biomolecules at a specific site so that the polymer can be manipulated by stimulation to alter ligand-biomolecule binding at an adjacent binding site, for example, the biotin binding site of streptavidin, the antigen-binding site of an antibody or the active, substrate-binding site of an enzyme. Binding may be completely blocked (i.e., the conjugate acts as an on-off switch) or partially blocked (i.e., the conjugate acts as a rheostat to partially block binding or to block binding only of larger ligands). Once a ligand is bound, it may also be ejected from the binding site by stimulating one (or more) conjugated polymers to cause ejection of the ligand and whatever is attached to it. Alternatively, selective partitioning, phase separation or precipitation of the polymer-conjugated biomolecule can be achieved through exposure of the stimulus-responsive component to an appropriate environmental stimulus.
REFERENCES:
patent: 4609707 (1986-09-01), Nowinski
patent: 4752638 (1988-06-01), Nowinski
patent: 4780409 (1988-10-01), Monji et al.
patent: 4783409 (1988-11-01), Tosa et al.
patent: 4839293 (1989-06-01), Cantor et al.
patent: 4843010 (1989-06-01), Nowinski
patent: 4946778 (1990-08-01), Ladner et al.
patent: 4971905 (1990-11-01), Holmes
patent: 5013669 (1991-05-01), Peters
patent: 5053228 (1991-10-01), Mori
patent: 5112770 (1992-05-01), Carbonell
patent: 5135876 (1992-08-01), Andrade et al.
patent: 5168049 (1992-12-01), Meade et al.
patent: 5226902 (1993-07-01), Bae et al.
patent: 5417983 (1995-05-01), Nagase et al.
patent: 5753261 (1998-05-01), Fernandez et al.
Orlandi et al., Proc. Natl. Acad. Sci. USA 86:3833-3837 (1989).
Paganelli et al., Int. J. Cancer 45:1184-1189 (1990).
Sambrook et al., Molecular Cloning: A Laboratory Manual, 2d Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sano et al., Bio/Tech. 11:201- (1993).
Takei, Y.G., et al., "Temperature-responsive Bioconjugates. 2. Molecular Design for Temperature-modulated Bioseparations", Bioconj. Chem., 4:341-346 (1993).
Walter et al., Anal. Biochem. 197:1-18 (1991).
Yarmush et al., Biotechnol. Prog. 8:168-178 (1992).
Achari et al., Biochemistry 31:10449-10457 (1992).
Akerstrom and Bjorck, J. Biol. Chem. 261:10240-10247 (1986).
Alexander et al., Biochemistry 31:3597-3603 (1992).
Bird et al., Science 242:423-426 (1988).
Burns et al., J. Org. Chem. 56:2648-2650 (1991).
Chen, et al., Biomaterials 11(9), 625-630 (Nov. 1, 1990).
Chen, et al. Biomaterials, 11(9), 631-634 (Nov. 1, 1990).
Chen, G.H. and A.S. Hoffman, "A new temperature-+and pH-responsive Copolymer for Possible Use in Protein Conjugation", Macromol. Chem. Phys., 196:1251-1259 (1995).
Chen, G.H., and A.S. Hoffman, "Preparation and Properties of Thermo-Reversible, Phase-Separating Enzyme-Oligo(NIPAAm) Conjugates", Bioconj. Chem. , 4:509-514 (1993).
Chen and Hoffman, "Synthesis of Carboxylated poly(NIPAAm) Oligomers and Their Application to Form Thermo-Reversible Polymer-enzyme Conjugates," J. Biomaterials Sci. Polymer Ed. 5:371-382 (1994).
Chen, G.H., and A.S. Hoffman, Nature 373:49-52 (1995).
Chen et al., ACS Polymer Preprints 33(2):468-469 (1992).
Chilkoti, A., et al., "Engineered Chimeric Streptavidin Tetramers as Novel Tools for Bioseparations and Drug Delivery," Biotechnology 13:1198-1204 (1995).
Chilkoti, A., et al., "Site-Specific Conjugation of a Temperature-Sensitive Polymer to a Genetically-Engineered Protein", Bioconj. Chem., 5:504-507 (1994).
Chilkoti, et al. Biocon. Chem. 5(6), 504-507 (Nov.-Dec. 1994).
Ciardelli, Biopolymers 23:1423-1437 (1984).
Davis et al., Bio/Technol. 9:165-169 (1991).
Derrick and Wigley, Nature 359:752-754 (1992).
Ding, Z.L., G.H. Chen, and A.S. Hoffman, "Synthesis and Purification of Thermally-Sensitive Oligomer-Enzyme Conjugates of Poly(NIPAAm)-Trypsin", Bioconj. Chem., 7:121-125 (1995).
Fujimura, M., T. Mori and T. Tosa, "Preparation and Properties of Soluble-Insoluble Immobilized Proteases", Biotech. Bioeng., 29:747-752 (1987).
Galaeu et al., J. Chromat., 684:37-43 (1994).
Green, Meth. Enzymol. 184:51-67 (1990).
Gronenborn et al., Science 253:657-661 (1991).
Harboe et al., J. Clin. Microbiol. 28:913-921 (1990).
Hendrickson et al., Proc. Natl. Acad. Sci. USA 86:2190-2194 (1989).
Heskins and Guillet, J. Macromol. Sci.-Chem. A2:1441-1455 (1968).
Hoffman, A.S., "Intelligent Polymers in Medicine and Biotechnology", Artif. Organs, 19:458-467 (1995).
Hoffman, A.S., "Intelligent Polymers in Medicine and Biotechnology", Macromol. Symp., 98:645-664 (1995).
Irie, M., "Light-induced Reversible Conformational Changes of Polymers in Solution and Gel Phase", ACS Polym. Preprints, 27(2):342-343 (1986).
Irie, M. and D. Kungwatchakun, "Photoresponsive Polymers. Mechanochemistry of Polyacrylamide Gels having Triphenylmethane Leuco Derivatives", Maokromol. Chem., Rapid Commun., 5:829-832 (1985).
Katz and Siewierski, J. Chromatogr. 624:403-409 (1992).
Kungwatchakun and Irie, Makromol. Chem., Rapid Commun. 9: 243-246 (1988).
Mamada et al., Macromolecules 23:1517-1519 (1990).
Martin et al., Meth. Enzymol. 203:121-153 (1991).
Matsuaka, M., et al., "Effect of Molecular Architecture of Poly(N-isopropylacrylamide)-trypsin conjugates on Their Solution and Enzymatic Properties," Bioconjugate Chem. 7:96-101 (1996).
Matsukata, M., et al., "Temperature Modulated Solubility-Activity Alterations for Poly(N-Isopropylacrylamide)-Lipase Conjugates", J. Biochem., 116:682-686 (1994).
Meyer et al., Exp. Hematol. 19:710-713 (1991).
Miura et al., Abstr. 17th Ann. Meet. Soc. Biomaterials (1991).
Miura et al., J. Biomater. Sci. Polymer Edn, 5(6):555-568 (1994).
Monji, N. C.A. Cole, and A. S. Hoffman, "Activated, N-Substituted Acrylamide Polymers for Antibody Coupling: Application to a Novel Membrane-Based Immunoassay", J. Biomtls. Sci. Polymer Ed., 5:407-420 (1994).
Monji, N., et al., "Application of a Thermally-Reversible Polymer-Antibody Conjugate in a Novel Membrane-Based Immunoassay", Biochem. and Biophys. Res. Comm., 172(2):652-660 (1990).
Monji, N. and A.S. Hoffman, "A Novel Immunoassay System and Bioseparation Process Based on Thermal Phase Separating Polymers", Appl. Biochem. and Biotech., 14:107-120 (1987).
Nguyen, A.L. and J.H.T. Luong, "Synthesis and Applications of Water-Soluble Reactive Polymers for Purification and Immobilization of Biomolecules", Biotech. Bioeng., 34:1186-1190 (1989).
Osada, Y. and S.B. Ross-Murphy, "Intelligent Gels", Sci. Amer., 268:82-87 (1993).
Otsu, T., et al. (1992) Eur. Polym. J., 28:1325-1329).
Padlan and Kabat, Meth. Enzymol. 203:3-21 (1991).
Park, T. G. and A. S. Hoffman, "Synthesis and Characterization of a Soluble, Temperature-Sensitive Polymer-Conjugated Enzyme", J. Biomtls. Sci. Polymer Ed., 4:493-504 (1993).
Phillips et al., Science 250:1130-1132 (1990).
Priest et al., ACS Symposium Series 350:255-264 (1987).
Sandmaier et al., Blood 76:630-635 (1990).
Shimoboji et al., Fifth World Biomaterials Congress May 29-Jun. 6, 1996.
Stappert et al., Nucleic Acid. Res. 20:624, (1992).
Stayton, P.S., et al., "Control of Protein-Ligand Recognition Using a Stimuli-Responsive Polymer", Nature , 378:472-474 (1995).
Takei, Y.G., et al., "Temperature-responsive Bioconjugates. 3. Antibody-Poly(N-isopropylacrylamide) Conjugates for Temperature-Modulated Precipitations and Affinity Bioseparations", Bioconj. Chem., 5:577-582 (1994).
Takei, Y.G. et al., "Temperature-res
Hoffman Allan S.
Stayton Patrick S.
Achutamurthy Ponnathapura
University of Washington
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