Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2008-04-08
2008-04-08
Achutamurthy, Ponnathapura N (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S069100, C435S183000, C435S195000, C435S320100, C536S023200
Reexamination Certificate
active
09868469
ABSTRACT:
Methods of producing cyclic peptides and splicing intermediates of peptides in a looped conformation are disclosed. The methods utilize the trans-splicing ability of split inteins to catalyze cyclization of peptides from a precursor peptide having a target peptide interposed between two portions of a split intein. The interaction of the two portions of the split intein creates a catalytically-active intein and also forces the target peptide into a loop configuration that stabilizes the ester isomer of the amino acid at the junction between one of the intein portions and the target peptide. A heteroatom from the other intein portion then reacts with the ester to form a cyclic ester intermediate. The active intein catalyzes the formation of an aminosuccinimide that liberates a cyclized form of the target peptide, which spontaneously rearranges to form the thermodynamically favored backbone cyclic peptide product. Also disclosed are nucleic acid molecules, polypeptides, methods for making cyclic peptides, methods of making libraries, and methods of screening peptides.
REFERENCES:
patent: 5643758 (1997-07-01), Guan et al.
patent: WO95/01800 (1995-01-01), None
patent: WO95/07351 (1995-03-01), None
patent: WO97/01642 (1997-01-01), None
Holford, M. et al: “Adding ‘splice’ to protein engineering”, STRUCTURE; vol. 6, Aug. 15, 1998, pp. 951-956, XP-000864581.
Southworth, M.W. et al: “Control of protein splicing by intein fragment reassembly”, EMBO Journal, vol. 17, No. 4, 1998, pp. 918-926, XP-002121550.
Mikheeva, S. et al: “Use of an engineered ribozyme to produce a circular human exon”, Nucleic Acids Research, vol. 25, No. 24, Dec. 1997, pp. 5085-5094, XP-002137476.
Wu, H. et al: “Proteintrans-splicing by a split intein encoded in a split DnaE gene ofSynechocystissp, PCC6803”, Proc. Natl. Acad. Sci. USA, vol. 95, Aug. 1998, pp. 9226-9231, XP-002137477.
Mills, K. et al: “Protein splicing intransby purified N—and C—terminal fragments of theMycobacterium tuberculosisRecA intein”, Proc. Natl. Acad. Sci. USA. vol. 95, Mar. 1998, pp. 3543-3548, XP002137478.
Scott, C. P. et al: “Production of cyclic peptides and protein in vivo”, Proc. Natl. Acad. Sci. USA, vol. 96, No. 24, Nov. 1999, pp. 13638-13643, XP002137479.
Evans, T.C. et al: “The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins”, Journal of Biological Chemistry, vol. 274, No. 26, Jun. 25, 1999, pp. 18359-18363. XP-002137480.
Iwai, H. et al: “Circular-beta-lactamase: stability enhancement by cyclizing the backbone”, FEBS LETTERS, vol. 459, No. 2, 1999, pp. 166-172, XP002137481.
Abel-Santos Ernesto V.
Benkovic Stephen J.
Scott Charles P.
Achutamurthy Ponnathapura N
Akerman & Senterfitt
Fronda Christian L.
The Penn State Research Foundation
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