Integration vector for Bradyrhizobium japonicum

Details Integration vector for Bradyrhizobium japonicum Integration vector for Bradyrhizobium japonicum

1987-10-07

1992-06-23

Naff, David M.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 935 29, 935 72, C12N 1500

Patent

active

051242605

ABSTRACT:
An integration vector which directs the stable integration of a DNA segment, including for example, a cloned structural gene and/or a selectable marker into a defined, nonessential region of the Bradyrhizobium japonicum genome is provided. The integration of the DNA occurs via homologous recombination between the DNA sequences which flank the DNA segment and identical sequences in the genome. The integration site is preferably the region of the B. japonicum genome flanked by the sequences RS.alpha.9 and RS.beta.3, near nifDK which is a nonessential region of the chromosome.

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