Integrated microarray analysis

Details Integrated microarray analysis Integrated microarray analysis

2008-04-29

2008-04-29

Martinell, James (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C435S194000, C435S195000

Reexamination Certificate

active

10526261

ABSTRACT:
The present invention relates to methods for identifying analyte nucleic acids present in a sample comprising nucleic acid molecules, said method comprising the steps of: (a) providing a porous substrate, said substrate comprising a plurality of micro-channels, wherein said micro-channels have immobilized thereon a target molecule capable of binding to an analyte present in said sample; wherein said channels are further provided with analyte amplification components, a reporter system; and said sample; (b) amplifying analyte nucleic acid molecules present within said sample; and (c) allowing binding to take place between an amplified analyte nucleic acid obtained in step (b), said target molecule immobilized onto the channels of said substrate and said reporter system, wherein said reporter system allows detecting whether binding has occurred between said target molecule and said analyte nucleic acid. The present invention further relates to the use of said methods as well as kits for carrying out said methods.

REFERENCES:
patent: 5691146 (1997-11-01), Mayrand
patent: 2004/0048270 (2004-03-01), Zeltz et al.
patent: 0 745 690 (1996-12-01), None
patent: WO 99/02266 (1999-01-01), None
patent: WO 00/31304 (2000-06-01), None
patent: WO 01/19517 (2001-03-01), None
patent: WO 01/31063 (2001-05-01), None
patent: WO 01/34842 (2001-05-01), None
patent: WO 01/48242 (2001-07-01), None
Belgrader et al., “Rapid Pathogen Detection Using a Microchip PCR Array Instrument.” Clinical Chemistry 44, 10:2191-2194 (1998).
Strizhkov et al., “PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations.” BioTechniques 29:844-857 (Oct. 2000).
Huber et al., “Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct: Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays.” Analytical Biochemistry 303:25-33 (2002).
Carmon et al., “Solid-Phase PCR in Microwells: Effects of Linker Length and Composition on Tethering, Hybridization, and Extension.” BioTechniques 32:410-420 (Feb. 2002).
van Beuningen et al., “Fast and Specific Hybridization Using Flow-Through Microarrays on Porous Metal Oxide.” Clinical Chemistry 47:1931-1933 (2001).
Ortiz et al., “PNA Molecular Beacons for Rapid Detection of PCR Amplicons.” Molecular and Cellular Probes 12:219-226 (1998).

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