Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving urea or urease
Patent
1991-10-17
1994-02-15
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving urea or urease
435 17, 435805, 422 56, 422 57, 436169, 436170, 526332, C12Q 158, C12Q 150, G01N 2177
Patent
active
052866246
ABSTRACT:
An integral multilayer analytical element for the determination of ammonia or an ammonia-producing substance comprising a light-transmissive liquid-impermeable support, an indicator layer containing an indicator which produces a detectable change by gaseous ammonia, a liquid permeation barrier layer, a reagent layer containing an alkaline buffer and optionally a reagent capable of reacting with a substance to produce ammonia and a spreading layer laminated in this order, which is improved by that the indicator layer contains a polyvinyl alkyl ether, and/or which is improved by that the surface of said support facing toward the indicator layer is undercoated with a polyvinyl alkyl ether, a hydroxyalkyl cellulose, an alkyl cellulose, polystyrene, a polyalkyl methacrylate, polyviriylidene chloride, polyvinyl alcohol or polyvinyl pyrrolidone, substantially not containing ammonia and ammonium ion. By using the above analytical element, ammonia or an ammonia-producing substance can be analyzed at a high coloring optical density and a high accuracy. The measuring accuracy is further improved by lowering the background optical density.
REFERENCES:
patent: 2799669 (1957-07-01), Zoss
patent: 4557901 (1985-12-01), Koyama
patent: 4604347 (1986-08-01), Arai
patent: 4895704 (1990-01-01), Arai
Encyclopedia of Polymer Science & Engineering vol. 17, John Wiley & Sons N.Y. 1989, pp. 456-457.
Kawase Tsuneo
Kitani Toru
Mori Toshihiro
Terashima Kaoru
Fuji Photo Film Co. , Ltd.
Gitomer Ralph
Wityshyn Michael G.
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