Intact gene and method of excising and cloning same

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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4351723, 935 9, 935 11, 536 27, C12P 1934, C12N 1500, C07H 1512

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047074450

ABSTRACT:
The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with mung bean nuclease and formamide under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products as m-RNA, S-RNA, t-RNA, polypeptides and the like.

REFERENCES:
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patent: 4554250 (1985-11-01), McCullough et al.
Doctor et al, In Methods in Enzymology, vol. XXIX, Chapter 29, pp. 419-431, 1974, Academic Press, Inc.
Maniatis et al., In Molecules Cloning: A Laboratory Manual, pp. 140-141, 270-294, 1982, Cold Spring Harbor Laboratory.
Britten et al, In Methods in Enzymology, vol. XXIX, Chapter 29, pp. 363-385, 1974, Academic Press, Inc.
McCutchaon et al., Science, vol. 225: 625-628, 1984.
A Microchemical Facility for the Analysis and Synthesis of Genes and Proteins, M. Hunkappiler, S. Kent, M. Caruthers, W. Dreyer, J. Firca, C. Giffin, S. Horvath, T. Humkapiller, P. Tempst & L. Hood Nature, vol. 310, Jul. 12, 1984, Division of Biology, California Institute of Technology, Pasadena, CA, 91125.

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