Insulin-dependent diabetes mellitus-specific chimeric polypeptid

Details Insulin-dependent diabetes mellitus-specific chimeric polypeptid Insulin-dependent diabetes mellitus-specific chimeric polypeptid

1996-01-26

1998-10-13

Chan, Christina

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

53038826, 435183, 930240, C07K 100, C07K 1400, C07K 1700, C12P 2108

Patent

active

058213341

ABSTRACT:
The present invention provides a chimeric polypeptide comprising an epitope of GAD65 protein and a structural region comprising a polypeptide of the GAD family, wherein the chimeric polypeptide is a more specific diagnostic for insulin dependent diabetes mellitus than intact GAD65 and produces fewer false positives than intact GAD65. The invention further provides a method of screening a subject for risk of developing IDDM, comprising contacting the chimeric polypeptide of claim 1 with a biological sample containing antibodies from the subject and detecting binding between an antibody in the biological sample and the chimeric polypeptide, the detection of binding indicating the subject is at risk of developing IDDM.

REFERENCES:
patent: 5200318 (1993-04-01), Rabin et al.
Solimena et al., J. of Cell Biology, vol. 126:331-341, Jul. 1994.
Daw et al. (Feb. 1995) Diabetes vol. 44(2):216-20.
Daw et al. (1996) J. Immunol. vol. 156(2):818-25.
Noriko Ujihara, Kendra Daw, Roberto Gianani, Esper Boel, Liping Yu, and Alvin C. Powers: Identification of Glutamic Acid Decarboxylase Autoantibody Heterogeneity and Epitope Regions in Type I Diabetes, Diabetes, 43:968-975 (Aug. 1994).
Wiltrud Richter, Yuguang Shi and Steinunn Baekkeskov: Autoreactive epitopes defined by Diabetes-Associated Human Monoclonal Antibodies . . . , Proc. Natl. Acad. Sci. USA 90:2832-2836 (Apr. 1993).
Wiltrud Richter, Josef Endl, Thomas H. Eiermann, Michael Brandt, Rosemarie Kientsch-Engel, Charles Thivolet, Herbert Jungfer and Werner A. Scherbaum: Human Monoclonal Islet Cell Antibodies From a Patient With Insulin-Dependent Diabetes Mellitus Reveal Glutamate Decarboxylase as the Target Antigen, Proc. Natl. Acad. Sci. USA 89:8467-8471 (Sep. 1992).

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