Insulin-binding peptides and uses thereof

Details Insulin-binding peptides and uses thereof Insulin-binding peptides and uses thereof

1987-04-09

1989-12-19

Moskowitz, Margaret

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

530328, 530329, 530350, 530351, 424110, C07K 740, C07K 706, A61K 3539

Patent

active

048884146

ABSTRACT:
The present invention concerns a novel class of peptides which specifically bind insulin. The diverse uses of these peptides relate to insulin, insulin action and the cellular receptor for insulin. The most preferred peptide of the invention is the hexapeptide-NH.sub.2 -cys-val-glu-glu-ala-ser-COOH which has marked affinity for the insulin B-chain.
Antibodies having specific binding affinity for peptides of the invention have identified and forseen uses. Such antibodies specifically directed toward a preferred peptide of the invention comprising cys-val-glu-glu-ala-ser represent a further preferred aspect of antibody-related aspects of the present invention.
In one significant aspect, the present invention involves a method for determining insulin levels in a biological fluid. A further aspect of the present invention involves a process for measuring insulin receptor sites involving the binding of antibodies to cells or cell fractions.
The present invention further comprises a method for decreasing the amount of biologically active insulin present in a biological fluid such as the blood of an animal. This method involves parenterally administering an amount of peptide of the invention in free or carrier-conjugated form.

REFERENCES:
patent: 3907765 (1975-09-01), Wang
patent: 4569791 (1986-02-01), Frank et al.
patent: 4569792 (1986-02-01), Frank et al.
patent: 4581165 (1986-04-01), Frank et al.
Rodionychev et al, "Delayed Hypoglycemic Activity of a Hexapeptide Analog Fragment 22-26 of Insulin B-Chain", Chemical Abstracts, vol. 93, p. 108, (161669k) 1980.
You-Min et al, "Studies on the Mechanism of Insulin Action Vi, The Interaction of Insulin Fragments with Insulin Receptor". Chemical Abstracts, vol. 93, p. 100 (161597k), 1980.
Kikuchi et al, "Studies on the Biological Activity of Degraded Insulins and Insulin Fragments", Chemical Abstracts, vol. 93, p. 77 (215776p), 1980.
Frank et al, "Modulation of Hepatic Insulin Receptors by a Human Growth Hormone Fragment (hGH6-13)", Chemical Abstracts, vol. 98, p. 144 (173579x), 1983.
Kikuchi et al, "Effect of Insulin Fragments on Biological Activity of Insulin and Desoctapeptide Insulin II Enhanced Binding and Mechanism Studies", Chemical Abstracts, vol. 95, p. 107 (181341s), 1981.
Mekler, (Biofizika, VIV (4), 1969, 581-584.
Idlis (Mendeleev Chem. J., 25:431-434).

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