Instrument setup system for a fluorescence analyzer

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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C250S459100

Reexamination Certificate

active

06897954

ABSTRACT:
The present invention provides instruments for analyzing a multiplicity of fluorescent dyes using a multiplicity of amplifying photodetectors, methods for using the instruments, methods for setting the instrument parameters, and methods for resetting the instrument parameters following a changed in photodetector amplification. The present invention is particularly applicable in the field of flow cytometry.

REFERENCES:
patent: 5314824 (1994-05-01), Schwartz
patent: WO 0128700 (2001-04-01), None
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Roederer, M. et al., “Eight Color, 10-Parameter Flow Cytometry to Elucidate Complex Leukocyte Hetrogeneity”, Cytometry 29, 328-339 (1997).
Shapiro et al, “A Flow Cytometer Designed for Fluorescence Calibration”, Cytometry 3, 280-287 (1998).
“BD FACSDiVa™ Option White Paper” Mar., 2002, (BD Biosciences, San Jose, CA; available at www.bdbiosciences.com).
Web Page: “Coulter Epics XL/XL-MCL Brochure p. 11”, © 1996, (Coulter International Corporation; downloaded Dec. 1, 2002 from http://scooter.cyto.purdue.edu/pucl_cd/flow/vol3/7/coulter/xl_mcl11.htm).
FACSComp™ Software User's Guide: Three-Color Applications, Jan. 1966, (BD Biosciences, San Jose, CA).
Bagwell, “Software Compensation Laboratory”, Presentation at the 25th Annual Course in Flow Cytometry: Research Methods and Applications, Jun. 8-14, 2002, Bowdoin College, Brunswick, Maine, USA.
Baumgarth, “A Practical Approach to Multicolor Flow Cytometry for Immunophenotyping”, Journal of Immunological Methods 243, 77-97 (2000).
RCA Corporation, Photomultiplier Handbook, 44-45 Copyright (1980).

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