Instrument for monitoring polymerase chain reaction of DNA

Details Instrument for monitoring polymerase chain reaction of DNA Instrument for monitoring polymerase chain reaction of DNA

2006-03-07

2006-03-07

Redding, David A (Department: 1744)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S288400, C435S288700, C422S082080, C356S072000

Reexamination Certificate

active

07008789

ABSTRACT:
An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

REFERENCES:
patent: 4284897 (1981-08-01), Sawamura et al.
patent: 4626684 (1986-12-01), Landa
patent: 4643877 (1987-02-01), Opitz et al.
patent: 4673289 (1987-06-01), Gaucher
patent: 4683202 (1987-07-01), Mullis
patent: 5073029 (1991-12-01), Eberly et al.
patent: 5091652 (1992-02-01), Mathies et al.
patent: 5169601 (1992-12-01), Ohta et al.
patent: 5215883 (1993-06-01), Chu
patent: 5243540 (1993-09-01), Van Albert et al.
patent: 5256880 (1993-10-01), Loree et al.
patent: 5315375 (1994-05-01), Allen
patent: 5355215 (1994-10-01), Schroeder et al.
patent: 5371016 (1994-12-01), Berndt
patent: 5383023 (1995-01-01), Walleczek
patent: 5389544 (1995-02-01), Sugata et al.
patent: 5475610 (1995-12-01), Atwood et al.
patent: 5547849 (1996-08-01), Baer et al.
patent: 5567947 (1996-10-01), Kebabian
patent: 5595708 (1997-01-01), Berndt
patent: 5656493 (1997-08-01), Mullis et al.
patent: 5672880 (1997-09-01), Kain
patent: 5736333 (1998-04-01), Livak et al.
patent: 5759781 (1998-06-01), Ward et al.
patent: 5766889 (1998-06-01), Atwood
patent: 5779978 (1998-07-01), Hartmann et al.
patent: 5792610 (1998-08-01), Witney et al.
patent: 5846842 (1998-12-01), Herron et al.
patent: 5854684 (1998-12-01), Stabile et al.
patent: 5872623 (1999-02-01), Stabile et al.
patent: 5926271 (1999-07-01), Couderc et al.
patent: 5943129 (1999-08-01), Hoyt et al.
patent: 6040940 (2000-03-01), Kawasaki
patent: 6057114 (2000-05-01), Akong et al.
patent: 6096272 (2000-08-01), Clark et al.
patent: 6197575 (2001-03-01), Griffith et al.
patent: 6287871 (2001-09-01), Herron et al.
patent: 6309601 (2001-10-01), Juncosa et al.
patent: 6331441 (2001-12-01), Balch et al.
patent: 6337740 (2002-01-01), Parce
patent: 6352672 (2002-03-01), Mabile et al.
patent: 6388788 (2002-05-01), Harris et al.
patent: 6411835 (2002-06-01), Modell et al.
patent: 6818437 (2004-11-01), Gambini et al.
patent: 2001/0033374 (2001-10-01), Hoyt
patent: 2002/0055178 (2002-05-01), Wardlaw
patent: 2002/0060791 (2002-05-01), Stumbo et al.
patent: 2002/0146688 (2002-10-01), Kinjo
patent: 0 065 409 (1982-11-01), None
patent: 0065409 (1982-11-01), None
patent: 0 640828 (1995-01-01), None
patent: 0 987 539 (2000-03-01), None
patent: 07-120392 (1995-05-01), None
patent: 07-120393 (1995-05-01), None
patent: 07-174701 (1995-07-01), None
patent: 09-281078 (1997-10-01), None
patent: WO 97/46707 (1997-11-01), None
patent: WO 01/35079 (2001-05-01), None
Wittwer, C.T., et al.,The LightCycler™: A Microvolume Multisample Fluorimeter with Rapid Temperature Control, Bio Techniquesvol. 22, No. 1, pp 176-181 (Jan. 1997).
Higuchi et al., “Kinetic PCR Analysis: Real-Time Monitoring Of DNA Amplification Reactions”, Bio Technology, vol. 11, pp. 1026-1030 (1993).
Ririe et al., “Product Differentiation By Analysis of DNA Melting Curves During the Polymerase Chain Reaction”, Analytical Biochemistry, vol. 245, pp. 154-160 (1997).

Affiliated with

Also associated with

Rating

Instrument for monitoring polymerase chain reaction of DNA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Instrument for monitoring polymerase chain reaction of DNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Instrument for monitoring polymerase chain reaction of DNA will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3564211