Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1987-11-03
1990-01-09
Niebling, John F.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
2041828, 435287, G01N 2728, G01N 2726
Patent
active
048926388
ABSTRACT:
An instrument for determination of the base sequence of deoxyribonucleic acid (DNA) which is designed to dectect DNA fragments in course of gel electrophoresis in real time to determine the base sequence of DNA, and comprises a gel electrophoretic panel part wherein DNA fragments labeled with a radioisotope are supplied to four places in a gel for four kinds of complementary strand synthesis reaction systems, respectively, and subjected to electrophoresis to form an electrophoretic pattern of the DNA fragments, and a detection part for detecting the electrophoretic pattern of the DNA fragments provided at a predetermined position in said gel electrophoretic panel part so as to have a position resolving power in a direction perpendicular to the direction of electrophoretic migration of the DNA fragments, and which instrument has a means for making the temperature of the gel in the vicinity of the detection part for detecting the electrophoretic pattern of the DNA fragments higher than that on the DNA fragment supply part side. By virtue of the provision of said means, the distance between two adjacent DNA bands similar to each other in molecular weight can be elongated, the resolving power for DNA bands can be improved, and the base sequence of nucleic acid can be determined with high precision.
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patent: 4679615 (1987-07-01), Livre
patent: 4707235 (1987-11-01), Englert et al.
Blasius & Augustin "Hochspannungspapierionophoretiche Trennug gemischter Cyamo-thiocyanatochromate (III) in Temperature-gradienten", Journal of Chromatography, 73 (1972) pp. 298-300.
Blasius & Klemm "Hochspannungspapierionophorese in Temperatur-gradienten und in Nicht wassrigen Losungmitteln", Journal of Chromatography, 108, No. 1, (1975) pp. 323-328.
Nagai Keiichi
Nakano Ryusei
Simada Tamotu
Sumitani Tomoaki
Tokita Jirou
Hitachi , Ltd.
Niebling John F.
Starsiak Jr. John S.
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