Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Patent
1995-06-07
1997-07-01
Redding, David A.
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
436173, 935 19, 935 77, G01N 3300, C12N 1500
Patent
active
056437986
ABSTRACT:
Improved techniques are provided for DNA sequencing, and particularly for sequencing of the entire human genome. Different base-specific reactions are utilized to use different sets of DNA fragments from a piece of DNA of unknown sequence. Each of the different sets of DNA fragments has a common origin and terminates at a particular base along the unknown sequence. The molecular weight of the DNA fragments in each of the different sets is detected by a matrix assisted laser absorption mass spectrometer to determine the sequence of the different bases in the DNA. The methods and apparatus of the present invention provide a relatively simple and low cost technique which may be automated to sequence thousands of gene bases per hour, and eliminates the tedious and time consuming gel electrophoresis separation technique conventionally used to determine the masses of DNA fragments.
REFERENCES:
patent: 4702808 (1987-10-01), Lemelson
patent: 4705616 (1987-11-01), Andersen et al.
patent: 4778993 (1988-10-01), Waugh
patent: 4849172 (1989-07-01), Yafuso et al.
patent: 4885077 (1989-12-01), Kamkelle et al.
patent: 4962037 (1990-10-01), Jett et al.
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
patent: 4999366 (1991-03-01), Yafuso et al.
patent: 5003059 (1991-03-01), Brennan
patent: 5064754 (1991-11-01), Mills
patent: 5135870 (1992-08-01), Williams et al.
patent: 5210412 (1993-05-01), Levis et al.
patent: 5221518 (1993-06-01), Mills
patent: 5288644 (1994-02-01), Beavis et al.
Article: "DNA Sequencing with Chain-terminating Inhabitors," Dec. 1977 By: Sanger et al.
Article: "Continuous, On-line DNA Sequencing Using Oligodeoxynucleotide Primers with Multiple Flurogphares," Aug. 1988, By: Brumbaugh et al.
Article: "Wiebers," Analytical Biochemistry, vol. 51, pp. 542-556, 1973.
Nature, vol. 293, pp. 270-275 (1981) By: Barber et al.
Article: "DNA Sequence Analysis: Past, Present, and Future," vol. 7, No. 5, (May 1989) By: Smith.
Chemical Abstracts, vol. 100, Abstract No. 100: 210331t, (1983) By: Tazawa et al.
Chemical Abstracts, vol. 103, Abstract No. 103: 61567g (1985) By: Benninghover.
Article: "Fast Atom Bombardment of Solids as an Ion Source in Mass Spectrometry", Nature, vol. 293, (24 Sep. 1981).
Beavis Ronald C.
Chait Brian T.
Redding David A.
The Rockefeller University
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