Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Patent
1992-11-13
1994-02-22
Housel, James C.
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
436173, 436807, 435 6, 935 77, 250282, G01N 3348, G01N 3356
Patent
active
052886441
ABSTRACT:
A method for DNA sequencing, and particularly for sequencing of the entire human genome. Different base-specific reactions are utilized to use different sets of DNA fragments from a piece of DNA of unknown sequence. Each of the different sets of DNA fragments has a common origin and terminates at a particular base along the unknown sequence. The molecular weight of the DNA fragments in each of the different sets is detected by a matrix assisted laser absorption mass spectrometer to determine the sequence of the different bases in the DNA. The methods and apparatus of the present invention provide a relatively simple and low cost technique which may be automated to sequence thousands of gene bases per hour, and eliminates the tedious and time consuming gel electrophoresis separation technique conventionally used to determine the masses of DNA fragments.
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Beavis Ronald C.
Chait Brian T.
Housel James C.
Redding David
The Rockefeller University
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