Instant culture media and method of sterilizing same

Details Instant culture media and method of sterilizing same Instant culture media and method of sterilizing same

1980-10-14

1982-05-11

Warden, Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

252399, 252401, 252405, 252407, 422 28, 422 30, 422 36, 422 37, 435243, 435254, 435255, 435256, 435800, C12N 120

Patent

active

043294316

ABSTRACT:
Disclosed are culture media comprising a gelling agent, germicidal bleach containing a halogen, a halogen-inactivating agent to inactivate residual halogen and conventional nutrients.
Also disclosed is a method for sterilizing a culture medium without autoclaving same by dissolving a gelling agent in buffered water; adding thereto a germicidal bleach containing a halogen; thereby forming a suspension; heating the suspension to boiling; adding to the stirred and heated suspension an excess of halogen-inactivating agent to neutralize residual halogen and then adding nutrients.

REFERENCES:
patent: 620022 (1899-02-01), Effront
patent: 2189949 (1940-02-01), Griffith et al.
patent: 3041250 (1962-06-01), Wolnak et al.
patent: 3293145 (1966-12-01), Leavitt et al.
patent: 4040977 (1977-08-01), Eggensperger et al.
patent: 4248971 (1981-02-01), Youssef

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