Instant culture media and method of sterilizing same

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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422 28, 422 30, 422 37, 435243, 435254, 435255, 435256, 435800, C12N 120

Patent

active

042489717

ABSTRACT:
Disclosed are culture media comprising an aqueous solution of a gelling agent, a compound such as a bleach capable of sterilizing the gelling agent and its container.
Also disclosed is a method for sterilizing a culture medium without autoclaving same by dissolving a gelling agent in boiling water; adding cool water to the aqueous gelling agent solution; dissolving a sterilizing agent in the cooled solution adding nutrients and then growing the culture.

REFERENCES:
patent: 620022 (1899-02-01), Effront
patent: 2189949 (1940-02-01), Griffith et al.
patent: 3041250 (1962-06-01), Wolnak et al.
patent: 3293145 (1966-12-01), Leavitt et al.
patent: 4040977 (1977-08-01), Eggensperger et al.
Martin Frobisher, Fundamentals of Microbiology, 8th Ed. W. B. Saunders Company; 1970 p. 41.

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