Installation for obtaining plasmids and cosmids

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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435300, 435310, 435311, 435287, 204299R, C12M 112, C12M 134, C12M 118, C12M 114

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active

053044886

ABSTRACT:
A process of separating plasmids and cosmids from bacterial cells in which they are contained, comprising adding cultured bacterial cells separated from a culture medium to an inclusion gel-forming substance; lysing the bacterial cells; solubilizing the thus lysed bacterial cells; solidifying the inclusion gel-forming substance to form a gel either before or after the step of solubilizing; and applying an electric field to the thus formed gel sufficient to cause electrophoretic migration of the plasmids and cosmids and isolation of the plasmids and cosmids. Also disclosed is a device for carrying out the process.

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patent: 4892638 (1990-01-01), Watanabe et al.
patent: 5139637 (1992-08-01), MacConnell
patent: 5188963 (1993-02-01), Stapleton
McClelland et al., "Restriction Endonucleases for Pulsed Field Mapping of Bacterial Genomes", Nucleic Acids Research, vol. 15, No. 15, pp. 5985-6005, 1987.
Ratlios et al., Curr. Sci., 58(22), 1235-39, 1989 Chemical Abstracts, vol. 112, No. 9, 73248C, 1990.
Kinashi et al., "Giant Linear Plasmids in Streptomyces Which Code for Antibiotic Biosynthesis Genes", Nature, vol. 328, pp. 454-456, Jul. 30, 1987.

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