Installation for obtaining plasmids and cosmids

Details Installation for obtaining plasmids and cosmids Installation for obtaining plasmids and cosmids

1992-11-13

1994-04-19

Housel, James C.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435300, 435310, 435311, 435287, 204299R, C12M 112, C12M 134, C12M 118, C12M 114

Patent

active

053044886

ABSTRACT:
A process of separating plasmids and cosmids from bacterial cells in which they are contained, comprising adding cultured bacterial cells separated from a culture medium to an inclusion gel-forming substance; lysing the bacterial cells; solubilizing the thus lysed bacterial cells; solidifying the inclusion gel-forming substance to form a gel either before or after the step of solubilizing; and applying an electric field to the thus formed gel sufficient to cause electrophoretic migration of the plasmids and cosmids and isolation of the plasmids and cosmids. Also disclosed is a device for carrying out the process.

REFERENCES:
patent: 3964992 (1976-06-01), Krotz
patent: 4716101 (1987-12-01), Thompson et al.
patent: 4735697 (1988-04-01), Burton
patent: 4818701 (1989-04-01), Littlehales
patent: 4892638 (1990-01-01), Watanabe et al.
patent: 5139637 (1992-08-01), MacConnell
patent: 5188963 (1993-02-01), Stapleton
McClelland et al., "Restriction Endonucleases for Pulsed Field Mapping of Bacterial Genomes", Nucleic Acids Research, vol. 15, No. 15, pp. 5985-6005, 1987.
Ratlios et al., Curr. Sci., 58(22), 1235-39, 1989 Chemical Abstracts, vol. 112, No. 9, 73248C, 1990.
Kinashi et al., "Giant Linear Plasmids in Streptomyces Which Code for Antibiotic Biosynthesis Genes", Nature, vol. 328, pp. 454-456, Jul. 30, 1987.

Affiliated with

Also associated with

Rating

Installation for obtaining plasmids and cosmids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Installation for obtaining plasmids and cosmids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Installation for obtaining plasmids and cosmids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-19327