Insertion of furin protease cleavage sites in membrane...

Details Insertion of furin protease cleavage sites in membrane... Insertion of furin protease cleavage sites in membrane...

2007-05-29

2007-05-29

Brumback, Brenda (Department: 1648)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

C435S006120, C435S463000, C435S219000, C424S093600

Reexamination Certificate

active

10841787

ABSTRACT:
Cleavage site for the protease furin is inserted between domains of a membrane glycoprotein. Upon cleavage by furin in the trans-Golgi network, the protein is separated into individual membrane-free domain that retains its native conformation. This protocol can be used to produce virus membrane protein domains for structural analysis and for trials as vaccines.

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