Insert for a issue culture vessel

Details Insert for a issue culture vessel Insert for a issue culture vessel

1994-08-15

1997-09-30

Warden, Robert J.

Chemistry: molecular biology and microbiology

Apparatus

422101, 4352971, 4352975, 4352992, 4353043, C12M 100, C12M 300

Patent

active

056725051

ABSTRACT:
An insert of the present invention for a tissue culture bottle with a neck includes a neck adapter with provision for mounting a suspending arm. One end of the suspending arm mounts in the insert and the other end has a holder for a specimen or a plurality of specimens. When the adapter is placed in the neck of the bottle, a specimen in the holder is suspended within the bottle. The neck adapter may also be incorporated into a cap for the bottle. In the case where the tissue culture bottle is a roller bottle with a longitudinal axis, the neck adapter may include rotatable elements so that the suspending arm and specimen holder remain in a substantially constant position with respect to the rotation of the bottle about the axis. In an alternate embodiment of the insert of the present invention, the insert may be a generally cylindrical member for placement on the interior surface of a tissue culture roller bottle for at least a portion of the cell growth period. The insert of the present invention may include a vessel to be used for addition of substances or withdrawal of substances to or from a tissue culture without substantially disturbing growing cells. The insert may be used to hold porous membranes having populations of cells for co-culture with a population of ceiling in a culture bottle. The insert may include ionic exchange resin, affinity membrane, and, when the holder includes a magnet, magnetic beads with biological affinity coatings. The insert may also incorporate a sensor for monitoring pH, concentration of other ions, temperature and the like. In any of these applications, the present invention allows the practitioner to conduct the desired activity with minimal disruption to growing cells and is generally applicable to standard tissue culture bottles and other laboratory equipment.

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