Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1993-07-07
1995-10-10
Furman, Keith C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
514 12, 514 21, C07K 14435
Patent
active
054571782
ABSTRACT:
This invention provides a family of insecticidally effective proteins and particular members of that family which may be isolated from the venom of the spider Filistata hibernalis, DNA encoding such proteins, insecticidal compositions of these proteins or the DNA encoding them, and methods for controlling invertebrate pests. Recombinant expression vectors and host cells and methods for producing insecticidally effective peptides are also provided.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4703008 (1987-10-01), Lin
patent: 4797279 (1989-01-01), Karamata et al.
patent: 4855405 (1989-08-01), Yoshioka et al.
patent: 4861595 (1989-08-01), Barnes et al.
patent: 4879236 (1989-11-01), Smith et al.
patent: 4918107 (1990-04-01), Nakajima et al.
patent: 4925664 (1990-05-01), Jackson et al.
Davies, et al., "Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteins", Biotech. 11:933-36 (1993).
Hink, et al., "Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines", Biotechnol. Prog., 7:9-14 (1991).
Skinner, et al., "Purification and characterization of two classes of neurotoxins from the funnel web spider, Agelenopsis aperta", J. Biol. Chem. 264:2150-55 (1989).
Stapleton, et al., "Curtatoxins: neurotoxic insecticidal polypeptides isolated from the funnel-web spider Hololena curta", J. Biol. Chem. 265:2054-2059 (1990).
Zlotkin et al., "An Excitatory and a Depressant Insect Toxin from Scorpion Venom both Affect Sodium . . . Site", Arch Biochem and Biophysics, 240:877-877 (1985).
Cutler "Electroporation being Developed to Transform Crops: Success with Model Crop Confirmed," AG Biotech. News vol. 7(5):3 & 17 (1990).
Tomalski et al., "Insect paralysis by baculovirus-mediated expression of a mite neurotoxin gene", Nature, 352:82-85 (1991).
Stewart et al., "Construction of an improved baculovirus insecticide containing an insect-specific toxin gene", Nature, 352:85-88 (1991).
McCutchen et al., "Development of a recombinant Baculovirus expressing an insect selective Neurotoxin: Potential for Pest Control," Biotechnology, 9:848-51 (1991).
Jackson and Parks, "Spider Toxins: Recent Applications in Neurobiology," Ann Rev Neurosci 12:405-14 (1989).
Adams, et al., "Isolation and Biological Activity of Synaptic Toxins from the Venom of the Funnel Web Spider, Agelenolpsis aperta," in Insect Neurochemistry and Neurophysiology 1986, Borkovec and Gelman eds., Humana Press, New Jersey, 1986.
Fugua, S. et al., "A simple PCR Method for detection and cloning low abundant transcript", Biotechnique, vol. 9, No. 2 (Aug. 1990).
Sambrook et al., "Molecular Cloning a Laboratory Manual", Second Ed. Cold Spring Harbor Press (1989).
Carbonell et al. "Synthesis of a gene coding for an insect-specific scorpion neurotoxin and attempts to express it using baculovirus vectors," Gene, 73:409-418 (1988).
Miller et al., "Bacterial, Viral, and Fungal Insecticides", Science, 219, 715-721 (1983).
Chomczynski et al., "Single-Step Method of RNA Isolation . . . Extraction", Analytical Biochemistry, 162, 156 (1987).
Saikki et al., "Primer-Directed Enzymatic Amplification of DNA . . . Polymerase", Science, 239:487 (1988).
Jones et al., "Molecular Cloning Regulation and Complete Sequence of a Hemocyanin-Related Juvenile Hormone-Suppressible Protein From Insect Hemolymphs", J. Biol. Chem. 265:8596 (1990).
Rossi et al., "An Alternate Method for Synthesis of Double-stranded DNA Segments," J. Biol. Chem. 257:9226 (1982).
Vialard, J. et al., "Synthesis of the Membrane Fusion and Hemagglutinin . . . Gene", J. Virology 64:37-50 (1990).
Summers and Smith, "A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures", Texas Agricultural Experiment Bulletin No. 1555, 1988.
Scopes, Anal. Biochem. 59:277 (1974).
Waddell, J. Lab. Clin. Med. 48:311 (1956).
T. V. Dunwiddie, "The Use of an In Vitro Brain Slices in Neuropharmacology", Electrophysiological Techniques in Pharmacology, H. M. Geller, 25ed. Alan R. Liss, Inc. New York (1986).
Jackson John R. H.
Johnson Janice H.
Kral, Jr. Robert M.
Krapcho Karen J.
FMC Corporation
Furman Keith C.
NPS Pharmaceuticals Inc.
LandOfFree
Insecticidally effective spider toxin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Insecticidally effective spider toxin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Insecticidally effective spider toxin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2310987