Insecticidal factor from field peas

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex

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A61K 3578

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active

059550821

ABSTRACT:
A method and composition is provided to control insect pests using pea extracts. Pea extracts, protein-rich, fibre-rich and starch-rich fractions were tested for toxicity against various grain storage pests. The pea protein-rich extract was found to be toxic against the lesser grain borer, red flour beetle, rice weevil, maize weevil, granary weevil and the rusty grain beetle. The pea protein-rich extract also reduced the number of offspring of all insects tested. The protein-rich fraction was also found to be effective against the bertha army worm, diamondback, grasshopper, Indian meal moth and flea beetle. A partially purified active extract from the protein-rich fraction has been identified, using ion-exchange chromatography, reversed-phase chromatography, adsorption chromatography and gel filtration. However, the active ingredient within this fraction is not a protein with a molecular weight>above 4,000 daltons, nor is it a protease inhibitor, lectin or soyasaponin I.

REFERENCES:
H.C.F. Su et al: Toxic effects of soybean saponin and its calcium salt on the rice weevil:, Journal of Economic Entomology, vol. 65, No. 3, 1972, College Park, Maryland, U.S., pp. 844-847, XP002065358, see p. 845, column 2, line-1-line 6.
Derwent Publications, Ltd. Database WPI: Section Ch, Week 7820; Class C03; AN78-35957A (XP002065359 & JP 53 038 626 A (Riken Koryo Kogyo K.).
Derwent Publications, Ltd. Database WPI: Section Ch, Week 8028; Class B04, AN80-49370C (XP002065360 & SU 698 623 A (Univ. Kish.).
The Effect of Yellow Split-Peas (Pisum Sativum L.) and Other Pulses on the Productivity of Certain Strains of Sitophilus Oryzae (L.) (Col. Curculionidae) and the Ability of Other Strains to Breed Thereon. C.W. Coombs et al., Ministry of Agriculture, Fisheries and Food, Pest Infestation Control Laboratory, London Road, Slough, Berks, England J. Stored Prod. Res. 1977, vol. 13, pp. 53-58, Pergamon Press, Printed in Great Britain.
The Potency and Effect of Phytotoxins Within Yellow Split-Pea (Pisum sativum) and adzuki bean (Vigna angularis) on survival and reproductive potential of Sitophilus oryzae (L.) (Coleoptera: Curculionidae) Graham, J. Holloway, Population Biology Laboratory, Department of Pure and Applied Zoology, University of Reading, Whiteknights, Reading, Berks, UK Bull. ent. Res. 76, pp. 287-295, Published 1986.
Toxins in Seeds E.A. Bell, Department of Plant Sciences, University of London, King's College, London, England, Biochemical Aspects of Plant and Animal Coevolution Proceedings of the Dhytochemical Soc. Symp. Reading, Apr. 1977, Harborne, J.B., pp. 143-161.

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