Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1989-01-23
1997-03-04
Chereskin, Che S.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800255, 800DIG27, 4353201, A01H 400
Patent
active
056081421
ABSTRACT:
A plant expression vector is constructed to cause the expression of an amino-terminal portion of the Bacillus thuringiensis delta-endotoxin gene in plant cells and the vector is used to create transgenic plants expressing the toxin. A truncated form of the toxin is used, with carboxy-terminal prolines added for stability. A translational enhancer sequence derived from the untranslated leader sequence from the mRNA of the coat protein gene of alfalfa mosaic virus coat protein gene is placed between a promoter and the toxin gene to increase translational efficiency. The transgenic plants produced are toxic to Lepidopteran pests and can transmit that trait to their progeny by normal Mendelian inheritance.
REFERENCES:
patent: 4448885 (1984-05-01), Schnepf et al.
patent: 4467036 (1984-08-01), Schnepf et al.
patent: 4820639 (1989-04-01), Gehrke
patent: 5159135 (1992-10-01), Umbeck
patent: 5254799 (1993-10-01), De Greve et al.
Gehrke et al. (1983) Biochemistry 22:5157-64.
Schnepf et al (1985) Journ. Biol. Chem. 260 (10):6273-80.
White et al. (1968) Principles of Biochemistry, Fourth Ed., Mc Graw-Hill Book Company, NY, p. 144.
Umbeck et al (Mar. 1987) Bio/Technology 5:263-266.
Hofte et al (Jun. 1989) Microbiological Reviews 53:242-255.
Vasil (Apr. 1988) Bio/Technology 6:397-402.
Schnepf et al (1985) Journal of Biological Chemistry 260:6264-6272.
Barton et al (1987) Plant Physiol. 85:1103-1109.
Vaeek et al (Jul. 1987) Nature 328:33-37.
Fischhoff et al (1987) Bio/Technology 5:807-813.
Knowles et al (1988) Molecular Microbiology 2(1):153-157.
Barton, et al., "Regeneration of Intact Tobacco Plants Containing Full Length Copies of Genetically Engineered T-DNA, and Transmission of T-DNA to Rl Progeny", Cell, vol. 32, 1033-1043 (Apr. 1983).
Gehrke, et al., "5'-Conformation of Capped Alfalfa Mosaic Virus Ribonucleic Acid 4 May Reflect Its Independence of the Cap Structure or of Cap-Binding Protein for Efficient Translation," Biochem., vol. 22, pp. 5157-5164 (1983).
Peralta, et al., "Overdrive, a T-DNA Transmission Enhancer on the A. Tumefaciens Tumour-Inducing Plasmid," EMBO Journal, vol. 5, No. 6, pp. 1137-1142 (1986).
Saiki, et al., "Enzymatic Amplification of B-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia," Science, vol. 230, pp. 1350-1354 (1985).
Schnepf, et al., "The Amino Acid Sequence of a Crystal Protein from Bacillus thuringiensis Deduced from the DNA Base Sequence," J. Biol. Chem., vol. 260, pp. 6264-6272 (1985).
Umbeck, et al., "Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants," Bio/Technology, vol. 5, No. 3, pp. 263-266 (1987).
Barton, K. A., et al., "Bacillus thuringiensis -Endotoxin Expressed in Transgenic Nicotiana tabacum Provides Resistance to Lepidopteran Insects," Plant Physiol., vol. 85, pp. 1103-1109 (1987).
Fischhoff, D. A., et al., "Insect Tolerant Transgenic Tomato Plants," Bio/Technology, vol. 5, pp. 807-813 (1987).
Gronenborn, B., et al., "Propagation of Foreign DNA in Plants Using Cauliflower Mosaic Virus As Vector," Nature, vol. 294, pp. 773-776 (1981).
Spear, Brian B., "Genetic Engineering of Bacterial Insecticides," Biotechnology In Agricultural Chemistry (Chapter 17), The American Chemical Society, pp. 204-214 (1987).
Vaeck, et al., "Transgenic Plants Protected from Insect Attack," Nature, vol. 328, pp. 33-37 (1987).
Aronson, A. I., et al., "Bacillus thuringiensis and Related Insect Pathogens," Microbiol. Rev., vol. 50, pp. 1-24 (1986).
Rowe, G. E. and A. Margaritis, "Bioprocess Deveopments in the Production of Bioinsecticides by Bacillus thuringiensis, " Crit. Rev. Biotechnol., vol. 6 pp. 87-127 (1987).
Sneh, B., et al., "Improvement of the Insecticidal Activity of Bacillus thuringiensis var. entomocidus on Larvae of Spodoptera littoralis (Lepidoptera, Noctuidae) By Addition of Chitinolytic Bacteria, a Phagostimulant and a UV-protectant," Z. Angew Entomol, vol. 96, pp. 77-83 (1983).
Whiteley, H. R. and H. E. Schnepf, "The Molecular Biology of Parasporal Crystal Body Formation in Bacillus thuringiensis, "Ann. Rev. Microbial, vol. 40, pp. 549-576 (1986).
Amann, E. and J. Brosius, "ATG Vectors for Regulated High-Level Expression of Cloned Genes in Escherichia coli, " Gene, vol. 40, pp. 183-190 (1985).
Bigelow, C. C. and M. Channon, "Hydrophobicities of Amino Acids and Proteins," CRC Handbook of Biochem. Mol. Biol., Ed. 3, vol. 1, pp. 209-243 (1982).
Blake, M. S., et al., "A Rapid, Sensitive Method for Detection of Alkaline Phosphatase-Conjugated Anti-antibody on Western Blots," Analytical Biochem., vol. 136, pp. 175-179 (1984).
Burnette, W. N., "`Western Blotting`: Electrophoretic Transfer of Proteins from Sodium Dodecyl Sulfate-Polyacrylamide Gels to Unmodified Nitrocellulose and Radiographic Detection With Antibody and Radioiodinated Protein A," Anal. Biochem., vol. 112, pp. 195-203 (1981).
Chirgwin, J. M., et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease," Biochem., vol. 24, pp. 5294-5299 (1979).
Dellaporta, S. L., et al., "Maize DNA Miniprep.", Mol. Biol. of Plants: A Lab. Course Manual, Cold Spring Harbor Lab., Cold Spring Harbor, NY, pp. 36-37 (1985).
Depicker, S. L., et al., "Nopaline Synthase" Transcript Mapping and DNA Sequence J. Mol. Appl. Genet., vol. 1, pp. 561-573 (1982).
Gallie, D. R., et al., "The 5'-Leader Sequence of Tobacco Mosaic Virus RNA Enhances the Expression of Foreign Gene Transcripts In Vitro and In Vivo," Nuc. Acids Res., vol. 15, pp. 3257-3273 (1987).
Gardner, R. D., et al., "The Complete Nucleotide Sequence of an Infectious Clone of Cauliflower Mosaic Virus by M13MP7 Shootgun Sequencing," Nucl. Acid Res., vol. 9, pp. 2871-2888 (1981).
Hood, E. E., et al., "The Hypervirulence of Agrobacterium tumefaciens A281 Is Encodes in a Region of pTiBo542 Outside of T-DNA," J. Bacteriol, vol. 168, pp. 1291-1301 (1986).
John, M. E., et al., "Identification and Characterization of cDNA Clones Specific for Cholesterol Side-Chain Cleavage Cytochrome P-450," Proc. Natl. Acad. Sci. USA, vol. 81, pp. 5628-5632 (1984).
Kozak, M., "Comparison of Initiation of Protein Synthesis in Procaryotes, Eucaryotes, and Organelles," Microbiol. Rev., vol. 47, pp. 1-45 (1983).
Murashige, T. and F. Skoog, "A Revised Medium For Rapid Growth and Bioassays With Tobacco Tissue Cultures," Physiol. Plant, vol. 15, pp. 473-497 (1962).
Ow, David, et al., "Functional Regions of the Cauliflower Mosaic Virus 35S. RNA Promoter Determined by Use of the Firefly Luciferase Gene as a Reporter of Promoter Activity," Proc. Natl. Acad. Sci. USA, vol. 84, pp. 4870-4874 (1987).
Yadav, N. S., et al., "Short Direct Repeats Flank the T-DNA on a Nopaline Ti Plasmid," Proc. Natl. Acad. Sci. USA, vol. 79, pp. 6322-6326 (Oct. 1982).
Barton Kenneth A.
Umbeck Paul F.
Agracetus, Inc.
Chereskin Che S.
LandOfFree
Insecticidal cotton plants does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Insecticidal cotton plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Insecticidal cotton plants will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2147476