Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1989-08-04
1992-10-13
Wax, Robert A.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352401, 4353201, 4351723, 43524021, 536 27, 935 32, 935 48, 935 70, C12N 500, C12N 1585, C12N 1587, C07H 1512
Patent
active
051550377
ABSTRACT:
The engineering of foreign vertebrate gene constructs by recombinant DNA techniques for the more efficient processing and secretion of foreign genes in insect systems is done by replacing the foreign vertebrate protein signal peptide sequences with protein signal sequences from insect cell secreted proteins. A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the insect signal peptides coding for the cuticle gene or adipokinetic hormone.
REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
patent: 4745051 (1988-05-01), Smith et al.
patent: 4879236 (1989-11-01), Smith et al.
Maeda et al. 1985, Nature 315, 592-594.
Ziegler et al. 1985, Biochem. Biophys. Res. Commun. 133, 337-342.
Schaffer et al. 1986, J. Cell. Biochem. Suppl. 10C, 56, Abs. K6.
Wallace et al. 1981, Nuc. Acids Res. 2, 879-894.
Heijne, et al. 1986 Nuc. Acids Res. 14, 4683-4690.
Baker et al. 1982, in: Fourth Edition The Study of Biology, Addison-Wesley Publ. Co., MA., pp. 905 & 907.
Nester et al. 1978, in: Microbiology Second Edition, Holt, Rinehart & Winston, New York, pp. 700-701.
Guarino et al. 1986, J. Virol. 57, 563-567.
Guarino et al. 1986, J. Virol. 60, 224-229.
Guarino et al. 1986, J. Virol. 60, 215-223.
Guarino et al. 1987, J. Virol. 61, 2091-2099.
Bradfield and Keeley, Adipokinetic Hormone Gene Sequence from Manduca sexta, The Journal of Biological Chemistry, vol. 264, No. 22, pp. 12791-12793 (Aug. 5, 1989).
Schultz-Allen et al., Synthesis of a Homodimer Neurohormone Precursor of Locust Adipokinetic Hormone Studied by In Vitro Translation and cDNA Cloning, Neuron, vol. 2 1369-1373 (1989).
M. Snyder, et al., Cuticle Protein Genes of Drosophila: Structure, Organization and Evolution of Four Clustered Genes, Cell, vol. 29, 1027-1040 (1982).
R. A. Fisher, et al., HIV infection is blocked in vitro by recombinant soluble CD4, Nature, vol. 331, 76-78 (1988).
R. E. Hussey, et al., A soluble CD4 protein selectively inhibits HIV replication and syncytion formation, Nature, vol. 331, 78-81 (1988).
P. J. Maddon, et al., The Isolation and Nucleotide Sequence of a cDNA Encoding the T Cell Surface Protein T4: A New Member of the Immunoglobin Gene Family, Cell, vol. 42, 93-104 (1985).
Susumu Maeda, Expression of Foreign Genes In Insects Using Baculovirus Vectors, Ann. Rev. Entomol., vol. 34, pp. 351-372 (1989).
Schaffer et al., Cloning of Adipokinetic Hormone cDNA, Society for Neuroscience Abstracts, vol. 14, Part 1, p. 29 (1988).
Schlultz et al., Molecular Biological Studies on the Synthesis of Locust Adipokinetic Hormones, Society of Neuroscience Abstracts, vol. 14, Part 1, p. 29 (1988).
Summers and Smith, A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures, Texas Agriculture Experiment Station Bulletin No. 1555, Texas A&M University, College Station, Tex. (1987).
K. Verner & G. Schatz, Protein Translocation Across Membranes, Science, vol. 241, 1307-1313 (Sep. 1988).
Low Christopher S. R.
The Texas A&M University System
Wax Robert A.
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