Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance
Reexamination Certificate
1998-03-11
2001-10-02
Benzion, Gary (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide confers pathogen or pest resistance
C536S023600, C435S252300, C435S320100, C800S294000
Reexamination Certificate
active
06297427
ABSTRACT:
FIELD OF THE INVENTION
The present invention provides the insect resistant use of sweet potato sporamin gene, and a method for controlling pests using the gene.
BACKGROUND OF THE INVENTION
In the past few years, according to the development of genetic engineering techniques, the introduction of genes encoding insect-resistant substances into plants by genetic techniques can enhance the insect-resistance of the transgenic plants. The insect-resistant substances include the toxic crystalline protein, protease inhibitor and the like produced by
Bacillus thuringiensis.
Sporamin is a storage protein enriched in the tuberous roots of sweet potato, which was first purified by Maeshima et al. (Maeshima et al., “Characterization of major proteins in sweet potato tuberous roots.”
Phytochemistry,
Vol. 124. pp. 1899-1902, 1985). The amount of Sporamin is about 60% to 80% of the total soluble proteins of sweet potato. Sporamin is present most in tuberous roots, little in other tissues and organs (Hattori et al., “High-level expression of tuberous root storage protein genes from sweet potato in stems of plantlets grown in vitro on sucrose medium.”
Plant Mol. Biol.
Vol. 14, pp. 595-604, 1990). In 1989, Hattori et al. isolated the sporamin gene from the cDNA library of tuberous roots of sweet potato (Hattori et al., “Structural relationship among the members of multigene family coding for the sweet potato tuberous roots storage proteins.”
Plant Mol. Biol.
Vol. 13, pp. 563-572, 1989).
Besides the function of storing nitrogen source, the amino acid sequence of sporamin predicted from the cDNA thereof has been found to have certain homology to Kunitz type trypsin inhibitors of Leguminosae plants (Hattori et al., “Sucrose-induced expression of genes coding for the tuberous root storage protein sweet potato in leaves and petioles.”
Plant Cell Physiol.
Vol. 32, pp. 79-86, 1991). However, Hattori et al. suggested that sporamin has no trypsin inhibitor activities (Hattori et al., 1989).
Moreover, with regard to the relationship between sweet potato sporamin gene and trypsin inhibitor activities was discussed by Chen, Jen-chin in “The research in the genome of sweet potato,” Dept. of Botany, National Taiwan University, Master thesis. pp. 1-122 (1994). It is suggested in the article that sporamin might possess trypsin inhibitor activity.
The purpose of the present invention is to find appropriate genes which can be transformed into plants and provide a method of controlling pests. According to the present invention, it is found that sporamin has insect-resistant ability, so it is possible to use sporamin to control pests.
SUMMARY OF THE INVENTION
The object of the present invention is to provide the use of sweet potato sporamin gene in insect-resistance.
The further object of the present invention is to provide a method for controlling pests, in which the sweet potato sporamin gene is transformed into a plant using the tobacco model to enhance the ability of insect resistance in the plant.
Still the further object of the present invention is to provide a transformation vector, in which the sweet potato sporamin gene is inserted, and then the sweet potato sporamin gene may be transformed into a plant to enhance the ability of insect resistance in that plant.
The present invention also provides a transformed bacterium, which is used as a vehicle to transfer the sweet potato sporamin gene into a plant to enhance the ability of insect resistance in that plant.
REFERENCES:
patent: 5306863 (1994-04-01), Hilder et al.
patent: 0 135343 (1985-03-01), None
Matsuoka, K. and K Nakamura, Propepetide of a precursor to a plant vacuolar protein required for vacuolar targeting PNAS, USA 88:834-838, 1991.*
Yeh, K. et al, Functional activity of sporamin from sweet potato (Ipomoea batatas Lam.): a tuber storage protein with trypsin inhibitory activity. Plant Mol Biol 33:565-570, Feb. 1997.*
Hattori, T. et al, Structural relationship among the members of a multigene family coding for the sweet potato tuberous protein. Plant Mol Biol 13:561-572, 1989.*
GenBank listing of Accession No. X15091, “Sweet potato mRNA for sporamin A tuberous root storage protein (clone pIM044)” Sep. 12, 1993.
Yeh, et al., (1997)Plant Cell Rep., vol. 16, No. 10, pp. 696-699.
Yeh et al., (1994)EMBL Sequence Database AccessionNo. U17333.
Yeh et al., (1997)Plant Mol. Biol., vol. 33, No. 3, pp. 565-570.
Wang et al., (1996)Taiwania,vol. 41, No. 1, pp. 27-34.
Rayn, C.A., (1990)Annual Review of Phytopathology, vol. 28, pp. 425-449.
Database WPI, Section Ch, Week 9119, Derwent Publication Ltd., Class D16, AN 91-136273 & JP 03072826 (Abstract).
Database WPI, Section Ch, Week 8645, Derwent Publication Ltd., Class B04, AN 86-29614 & JP 61219388 (Abstract).
Lin, Mei-In, (Oct. 1996)Study on Gene Expression and Insect-Resistance of Sweet Potato Sporamin in Transgenic Tobacco,Master Thesis (Abstract) National Tiawan University.
Chen Yih-Ming
Kao Suey-Sheng
Lin Chu-Yung
Lin Mei-In
Tuan Shu-Jen
Benzion Gary
Kruse David H
McDonnell & Boehnen Hulbert & Berghoff
National Science Council
LandOfFree
Insect resistant use of sweet potato sporamin gene and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Insect resistant use of sweet potato sporamin gene and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Insect resistant use of sweet potato sporamin gene and... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2591497