Insect desiccation resistance genes and uses thereof

Details Insect desiccation resistance genes and uses thereof Insect desiccation resistance genes and uses thereof

2011-05-10

2011-05-10

Desai, Anand U (Department: 1656)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100, C435S069100, C435S325000, C435S440000

Reexamination Certificate

active

07939647

ABSTRACT:
An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced fromPolypedilum vanderplankilarvae in a desiccated state, aP. vanderplankiEST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins. When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from these genes were heat soluble even when boiling, so that these proteins have hydrophilic properties as high as plant LEA proteins. Therefore, the three isolated genes were found to be novelP. vanderplanki-derived LEA genes. Furthermore, introduction of these genes into animal cells successfully conferred desiccation resistance to the cells.

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