Insect control with multiple toxins

Details Insect control with multiple toxins Insect control with multiple toxins

1995-05-08

1998-05-26

Wax, Robert A.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

4351721, 4351723, 4353201, 424 932, 424 933, 424 936, C12N 701, A01N 6300

Patent

active

057563403

ABSTRACT:
A method is provided that accelerates the rate of kill of pests such as from the order Lepidoptera. The method comprises treating the pests or their loci with at least two different insect toxins which are expressed from at least one recombinant microbe. Pairs of toxins that do not compete with each other on the same binding site and that differ in their pharmacology have been found to provide synergistic control. Preferred insecticidal microbes are baculoviruses.

REFERENCES:
patent: 4668511 (1987-05-01), Aspirot et al.
patent: 4745051 (1988-05-01), Smith et al.
patent: 4762547 (1988-08-01), Iwasaki et al.
patent: 4870023 (1989-09-01), Fraser et al.
patent: 4888340 (1989-12-01), Neh et al.
patent: 4929718 (1990-05-01), Possani et al.
patent: 5071748 (1991-12-01), Miller
patent: 5098706 (1992-03-01), Hammock et al.
patent: 5162308 (1992-11-01), Brown et al.
patent: 5177308 (1993-01-01), Barton et al.
patent: 5180581 (1993-01-01), Miller et al.
patent: 5238724 (1993-08-01), Bjostad, III et al.
patent: 5266314 (1993-11-01), Maeda
patent: 5266317 (1993-11-01), Tomalski et al.
patent: 5457178 (1995-10-01), Jackson et al.
Trainer et al. (1993) J. Biol. Chem 268:17114-17119 "Neuntoxin Binding and Allosteric Modulation at Receptor Sites 2 and 5 on purified and . . . ".
Moffat, Anne Simon, "New Chemicals Seek to Outwit Insect Pests," Science 261, pp. 550-551 (1993).
Hammock et al., "The Role of Juvenile Hormone Metabolism in the Metamorphosis of Selected Lepidoptera," Chemical Abstracts, 102 (1985), entry 76006b.
Abdel-Aal and Hammock, "3-Octylthio-1,1,1-trifluoro-2-propanone, A High Affinity and Slow Binding Inhibitor of Juvenile Hormone Esterase from Trichoplusia ni (Huber)," Insect Biochem., 15:1 (1985), pp. 111-122.
Abdel-Aal and Hammock, "Transition State Analogs as Ligands for Affinity Purification of Juvenile Hormone Esterase," Science , 233 (Sep. 1986), pp. 1073-1076.
Bachmair and Varshavsky, "The Degradation Signal in a Short-Lived Protein," Cell, 56 (Mar. 1989), pp. 1019-1032.
Cheung and Hammock, "Micro-Lipid-Droplet Encapsulation of Bacillus thuringiensis subsp. israelensis .delta.-Endotoxin for Control of Mosquito Larvae," Appl.. & Environ. Microlbiol., 50:4 (Oct. 1985), pp. 984-988.
Chiang and Dice, "Peptide Sequences that Target Proteins for Enhanced Degradation During Serum Withdrawal," J. of Biol. Chem., 263:14 (May 1988), pp. 6797-6805.
Hammock and Sparks, "A Rapid Assay for Insect Juvenile Hormone Esterase Activity," Analytical Biochemistry, 82 (1977), pp. 573-579.
Hammock et al., "Expression and Effects of the Juvenile Hormone Esterase in a Baculovirus Vector," Nature, 344:6265 (Mar. 1990), pp. 458-461.
Hammock and Rose, "Analysis of Juvenile Hormone Esterase Activity," Chpt. 32, pp. 487-495 in Law et al. (Eds.), Methods in Enzymology, vol. III: Steroids and Isoprenoids (Part B), Academic Press (1985).
Hammock et al., "Trifluoromethylketones as Possible Transition State Analog Inhibitors of Juvenile Hormone Esterase," Pesticide Biochem. & Physiology, 17 (1982), pp. 76-88.
Hammock et al., "Selective Inhibition of JH Esterases from Cockroach Hemolymph," Pesticide Biochem. & Physiology, 7 (1977), pp. 517-530.
Hammock et al., "Strategies for the Discovery of Insect Control Agents: . . . " Chpt. 12 in Steffens et al. (Eds), Biomechanism Regulating Growth & Development, USDA Beltsville Symp. vol. 12, Kluwer Academic Press (1988).
Hanzlik et al., "Isolation and Sequencing of cDNA Clones Coding for Juvenile Hormone Esterase from Heliothis virescens, " J. of Biol. Chem., 264:21 (Jul. 1989), pp. 12419-12425.
Hanzlik and Hammock, "Characterization of Affinity-purified Juvenile Hormone Esterase from Trichoplusia ni," J. Biol. Chem., 1987:23 (Oct. 1987), pp. 13584-13591.
Huynh et al., "Constructing and Screening cDNA Libraries in .lambda.gt10 and .lambda.gt11," Chpt. 2, IRL Press (Oxford), (1985), pp. 49-78.
Ichinose et al., Pharmacokinetic Studies of the Recombinant Juvenile Hormone Esterase in Manduca sexta, Pepticide Biochem. & Physiology, 42 (1992), pp. 13-23.
Ichinose et al., "Uptake of Juvenile Hormone Esterase by Pericardial Cells of Manduca sexta," Insect Biochem. Molec. Biol. (1992). 22(8): 893-904.
McCutchen et al., "Development of a Recombinant Baculovirus Expressing an Insect-Selective Neurotoxin: Potential for Pest Control," Bio/Technology, 9 (Sep. 1991), pp. 848-852.
Philpott and Hammock, "Juvenile Hormone Esterase is a Biochemical Anti-Juvenile Hormone Agent," Insect Biochem., 20:5 (1990), pp. 451-459.
Rogers et al., "Amino Acid Sequences Common to Rapidly Degraded Proteins: The PEST Hypothesis," Science, 234 (Oct. 1986), pp. 364-368.
Sparks and Hammock, "Induction and Regulation of Juvenile Hormone Esterases During the Last Larval Instar of the Cabbage Looper, Trichoplusia ni, " J. Insect. Physiolo., 25 (1979), pp. 551-560.
Sparks and Hammock, "Comparative Inhibition of the Juvenile Hormone Esterases form Trichoplusia ni, Tenebrio molitor, and Musca domestica, " Pesticide Biochem. & Physiology, 14 (1980), pp. 290-302.
Wozniak and Jones, "Immunochemical Characterization of Juvenile Hormone Esterase from Different Species of Lepidoptera," Biochem. & Biophys. Res. Commun., 144:3 (May 1987), pp. 1281-1286.
Wrobleski et al., "Regulation of Juvenile Hormone Esterase Gene Expression in the Tobacco Budworm (Heliothis virescens)," Archives of Biochem. & Biophys., 278:2 (May 1990), pp. 461-466.
Elridge et al., "Insecticidal Properties of Genetically Engineered Baculoviruses Expressing an Insect Juvenile Hormone Esterase Gene," Appl. & Environ. Microbiol., 58:5 (May 1992), pp. 1583-1591.
Hayakawa, "Structure of a Growth-Blocking Peptide Present in Parasitized Insect Hemolymph," J. of Biol. Chem., 266:13 (May 5, 1991), pp. 7982-7984.
Hayakawa, "A Putative New Juvenile Peptide Hormone in Lepidopteran Insects," Biochemical and Biophysical Research Communications, 185:3 (Jun. 30, 1993), pp. 1141-1147.
Smith et al., "Production of Human Beta Interferon in Insect Cells Infected with a Baculovirus Expression Vector," Mol. Cell Biol., 3 (1983), pp. 2156-2165.
Betana et al., "Potential of Baculo Viruses Expressing a Scorpion Toxin and an Esterase in Agriculture . . . ," Abstr. Pap. Am. Chem. Soc., (206 Meet., Pt. 1, AGR0122), 1993 (Abstract only).
Hayakawa, "Juvenile Hormone Esterase Activity Repressive Factor in the Plasma of Parasitized Insect Larvae," J. Biol. Chem., 265:19 (1990), pp. 10812-10816. (Abstract only).
Ward et al., "Analysis of the Catalytic Mechanism of Juvenile Hormone Esterase by Site-Directed Mutagenesis," Int. J. Biochem. (England), 24:12 (Dec. 1993), pp. 1933-1941 (Abstract only).
Hammock et al., "Development of Recombinant Viral Insecticides by Expression of an Insect-Speicfic Toxin . . . ," Arch. Insect Biochem. Physiol. (US), 22:3-4 (1993), pp. 315-344 (Abstract only).
Possee et al., "Expression of the Proteins with Insecticidal Activities Using Baculo Virus Vectors . . . ," Ann. N.Y. Acad. Sci., 646 (1991), pp. 234-239 (Abstract only).
Hammock et al., "Improving the Efficacy of Baculo Virus Insecticides by Expressing with Insect Selective Proteins," Abstr. Pap. Am. Chem. Soc. (202 Meet., Pt. 1, AGR09) (1991) (Abstract only).
Bonning et al., "Further Development of a Recombinant Baculovirus Insecticide Expressing the Enzyme JHE from Heliothis-Virescens," Biochem. Mol. Biol., 22:5 (1992) pp. 453-458 (Abstract only).
Piek et al., "The Pharmacology of Microbracon Venom," Comp. Biochem. Physiol., vol. 72C, pp. 303-309 (1982).
Miller et al., "Bacterial, Viral, and Fungal Insecticides," Science, 219, pp. 715-721, (Feb. 11, 1983).
Sakurai et al., "Complete Nucleotide Sequence of Gene for Sex-Specific Storage Protein of Bombyx mori," Nucleic Acids Research, 16:15, pp. 7717-7718 (1988).
Merryweather et al., "Construction of Genetically Engineered Baculovirus Insecticides Containning the Bacillus thuringiensis subsp. kurstaki HD-73 Delta Endotoxin," J. of Gen. Virol., 71, pp. 1535-1544 (1990).
Martens et al., "Insecticidal Activity of a Bacterial Crystal Protein Expressed by a Recombinant Baculovirus in Insect Cells," Applied and Environmental Microbiology, 56:9, pp.

Affiliated with

Also associated with

Rating

Insect control with multiple toxins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Insect control with multiple toxins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Insect control with multiple toxins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1959097