Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1995-05-30
1996-06-25
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
4351721, 4351723, 435134, 435194, 4352542, 43525421, 4353201, 536 231, 536 232, 536 237, C12P 702, C12P 764, C12N 912, C12N 119
Patent
active
055299121
ABSTRACT:
A yeast cell, preferably Saccharomyces cerevisiae, which contains a functional stable recombinant DNA sequence that does not allow for the encoding of a negative regulator of phospholipid biosynthesis therein and which has multiple copies of an INO1 gene. In a preferred embodiment, the recombinant DNA sequence is an OPI1 gene deletion which results in the deregulation of inositol or inositol-containing metabolites such as inositol-1-phosphate synthase. Moreover, there is a method for obtaining inositol, inositol-containing metabolites or phospholipids such as myo-inositol or inositol-1-phosphate. The method comprises the steps of genetically engineering a stable yeast cell, preferably Saccharomyces cerevisiae, to continually produce inositol, inositol-containing metabolites or phospholipids. Additionally, there is the step of then generating the inositol, inositol-containing metabolites or phospholipids. In a preferred embodiment, the genetically engineering step includes the step of altering the negative regulatory step involved in phospholipid biosynthesis to overproduce inositol, inositol-containing metabolites or phospholipids such as myo-inositol.
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Hirsch et al. Genet. 116, Suppl. No. 1, pt. 2:551, abstract B.21 (1987).
Hirsch et al. Mol. Cell. Biol. 6:3320 (1986).
Henry Susan A.
White Michael J.
Carnegie Mellon University
LeGuyader John L.
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