Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-05-14
1994-10-25
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 29, 435 34, 435975, 436 64, 436166, C12Q 132
Patent
active
053588550
ABSTRACT:
A cyto(histo)chemical technique for determining inosine 5'-monophosphate dehydrogenase (IMPD) presence and concentration within one or more morphologically classified or classifiable cells taken from an animal or a human subject. Cell monolayers are prepared, fixed and stained according to a specified procedure in which inosine 5'-monophosphate (as enzyme substrate), nicotinamide adenine dinucleotide (NAD), phenazine methosulfate (PMS) and nitro blue tetrazolium (NBT) as a diazonium salt together produce a granular, dark blue deposit of formazan generally proportional to the IMPD activity in the cell. Cells are evaluated either visually or by image analysis, with a computerized image analyzer or other imaging system, according to accepted biostatistical methods and standards.
REFERENCES:
patent: 3925154 (1975-12-01), Enei et al.
patent: 4254222 (1981-03-01), Owen
patent: 4351899 (1982-09-01), Owen
patent: 4427771 (1984-01-01), Misaki et al.
patent: 4629697 (1986-12-01), Limbach et al.
patent: 4683198 (1987-07-01), Ishikawa et al.
patent: 4849347 (1989-07-01), Familletti et al.
patent: 4916061 (1990-04-01), Di Ianni
patent: 5036000 (1991-07-01), Palmer et al.
Negishi et al., Reagent for spectrometric determination of inosinic acid, Japan, Kokai Tokkyo Koho, 12 pp., Oct. 12, 1989, Heisei.
Henderson et al., Inhibitors of Inosinate dehydrogenase activity prog. chemother, proc., Int. Congr. chemother., 8th Meeting Date 1973, vol. 3, 152-4.
Jayaram, Cytotoxicity of New IMP dehydrogenase, Biochem. Biophys. Res. Commun., 186(3), 1600-6, 1992.
Yamada et al., Two distinct target sites on IMP dehydrogenase Adv. Exp. Med. Biol., 253B (Purine Pyrimidine Metab. Mang. Pt. B).
Markovic Nenad S.
Markovic Olivera T.
Markovic Svetomir N.
Puglia Charles D.
Roberts Jay
Housel James C.
Pyon Harold Y.
The Medical College of Pennsylvania
LandOfFree
Inosinic acid dehydrogenase assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Inosinic acid dehydrogenase assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inosinic acid dehydrogenase assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-134687