Inorganic carrier containing bound silane coupling agent having

Details Inorganic carrier containing bound silane coupling agent having Inorganic carrier containing bound silane coupling agent having

1997-04-24

1999-12-21

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

435134, 435181, C12N 1114, C12N 1106, C12P 764

Patent

active

060047860

ABSTRACT:
A carrier for immobilizing lipase is prepared by bonding a silane coupling agent having a carboxylic-ester group to an inorganic carrier. Preferred silane coupling agents are .gamma.-methacryl oxypropyltrimethoxy silane, .gamma.-acetoxypropyltrimethoxy silane or .gamma.-acryloxypropyltrimethoxy silane. The inorganic carrier can be kaolinite, porous glass, bentonite, silica gel, alumina, silica, silica-alumina hydroxy apatite or calcium phosphate gel. Lipase is immobilized by mixing lipase with the inorganic carrier containing the bound silane coupling agent, filtering the resultant mixture and drying the filtered mixture. Functional groups of the lipase are bonded to the carboxylic-ester group of the silane coupling agent.

REFERENCES:
patent: 4384045 (1983-05-01), Ho et al.
patent: 4415631 (1983-11-01), Schutijser

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