Injection method for microfluidic chips

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S604000

Reexamination Certificate

active

07927476

ABSTRACT:
Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.

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