Injecting Drosophila embryos

Details Injecting Drosophila embryos Injecting Drosophila embryos

2007-11-20

2010-06-08

Chen, Shin-Lin (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

Via microinjection of dna into an embryo, egg cell, or...

C536S023100, C435S455000

Reexamination Certificate

active

07732659

ABSTRACT:
The present invention provides systems that allow reliable multiplexed transformation ofDrosophilaembryos. The present invention provides methods and reagents that allow preparation of injection-quality nucleic acid samples and that allow simultaneous preparation of multiple such samples. The present invention provides systems for simultaneous processing of multiple injected embryos. The present invention provides methods for transformation ofDrosophilaembryos involving use of virginator strains that can be used to increase the efficiency of setting up the crosses needed to produce the eggs for the injections and for the crosses needed to screen for transformants.

REFERENCES:
patent: 5977089 (1999-11-01), Arimilli
patent: 6005087 (1999-12-01), Cook
patent: 6031086 (2000-02-01), Switzer
patent: 6127533 (2000-10-01), Cook
patent: 6225460 (2001-05-01), Bischofberger
patent: 6399754 (2002-06-01), Cook
patent: 6403779 (2002-06-01), Kawasaki
patent: 6593129 (2003-07-01), Takeshita et al.
patent: 2003/0175950 (2003-09-01), McSwiggen
patent: 2004/0092470 (2004-05-01), Leonard et al.
patent: 2004/0192626 (2004-09-01), McSwiggen et al.
patent: 2005/0020525 (2005-01-01), McSwiggen et al.
patent: 2005/0032733 (2005-02-01), McSwiggen et al.
patent: 2006/0240409 (2006-10-01), Prince et al.
Baldarelli et al., 1990, Nucleic Acids Research, vol. 18, No. 19, 5903-5904.
Robertson et al., 1988, Genetics, vol. 118, p. 461-470.
Adams, et al.,Science, 287:2185-2195, 2000.
Bateman, et al.,Genetics, 173:769-777, 2006.
Beames, et al.,Virology, 174:354, 1990.
Bellen, et al.,Genetics, et al., 167:761-781, 2004.
Berg, et al.,Genetics, 127:515-524, 1991.
Bischof, et al.,Proc. Natl. Acad. Sci., USA, 104:3312-3317, 2007.
Celniker, et al.,Genome Biol., 3:RESEARCH0079, 2002.
Clark, et al.,Mol. Biol. Evol., 11:40-50, 1994.
Copeland, et al.,Nat. Rev. Genet., 2:769-779, 2001.
Daniels, et al.,Genetics, 124:339-355, 1990.
Elick, et al.,Genetica, 97:127-139, 1995.
Fish, et al.,Nat. Protocols, 2:2325-2331, 2007.
Fraser, et al.,Virology, 211:397-407, 1995.
Gelsthorpe, et al.,Genetics, 174:265-270, 2006.
Golic, et al.,Nuc. Acid. Res., 25:3665-3671, 1997.
Grether, et al.,Genes and Development, 9:1694-1708, 1995.
Grinblat, et al.,Development, 120:91-102, 1994.
Groth, et al.,Genetics, 166:1775-1782, 2004.
Groth, et al.,Proc. Natl. Acad. Sci., USA, 97:5995-6000, 2000.
Haenlin, et al.,Cell, 40:827-837, 1985.
Hagemann, et al.,Mol. Gen. Genet., 244:168-175, 1994.
Hall,Drosophila Inform. Serv., 50:103, 1973.
Hoskins, et al.,Science, 287:2271-2274, 2000.
Kimmerly, et al.,Genome Res., 6:414-430, 1996.
Lansman, et al.,Proc. Natl. Acad. Sci., USA, 84:6491-6495, 1987.
Lee, et al.,Genomics, 73:56, 2001.
Levis, et al.,Science, 229:558-561, 1985.
Lisi, et al.,Genetics, 154:669-678, 2000.
McEwen, et al.,Development, 132:3935-3946, 2005.
Miller, et al.,Biotechniques, 33:366-367, 2002.
Miller, et al.,Proc. Natl. Acad. Sci., USA, 89:4018-4022, 1992.
Neuburger, et al.,Genetics, 173:1377-1387, 2006.
Norga, et al.,Curr. Biol., 13:1388-1396, 2003.
O'Kane, et al.,Proc. Natl. Acad. Sci., 84:9123-9127, 1987.
Olivares, et al.,Nat. Biotechnol., 20:1124-1128, 2002.
Ortiz-Urda, et al.,Hum. Gene Ther., 14:923-928, 2003.
Ortiz-Urda, et al.,J. Clin. Invest., 111:251-255, 2003.
Ortiz-Urda, et al.,Nat. Med., 8:1166-1170, 2002.
Paricio, et al.,Nucleic Acids Res., 19:6713-6718, 1991.
Perkins, et al.,Proc. Natl. Acad. Sci., USA, 89:10753-10757, 1992.
Rong, et al.,Genes Dev., 16:1568-1581, 2002.
Rong, et al.,Genetics, 157:1307-1312, 2001.
Rong, et al.,Science, 288:2013-2018, 2000.
Rubin, et al.,Science, 218:348-353, 1982.
Shizuya, et al.,Proc. Natl. Acad. Sci., USA, 89:8794-8797, 1992.
Siegal, et al.,Genetics, 144:715-726, 1996.
Siegal, et al.,Methods Mol. Biol., 136:487-495, 2000.
Spradling, A. (1986) P-element mediated transformation inDrosophila, pp. 175-198 A Practical Approach by D. B. Roberts IRL Press.
Spradling, et al.,Genetics, 153:135-177, 1999.
Spradling, et al.,Science, 218:341-347, 1982.
Sternberg, et al.,Proc. Natl. Acad. Sci., USA, 87:103-107, 1990.
Thorpe, et al.,Proc. Natl. Acad. Sci., USA, 95:5505-5510, 1998.
Thyagarajan, et al.,Mol. Cell. Biol., 21:3926-3934, 2001.
Vagin, et al.,Science, 313:320-324, 2006.
Venken, et al.,Science, 314:1747-1751, 2006.
Wang, et al.,Insect. Mol. Biol., 1:109-116, 1993.
Wild, et al.,Genome Res., 12:1434-1444, 2002.
Wright, et al.,Drosophilia Information Service, 87:108, 1974.
Baldarelli et al. “Transient expression of DNA after ballistic introduction intoDrosophiliaembryos” Nucleic Acids Research, vol. 18, No. 19, pp. 5903-5904, Aug. 27, 1990.
International Search Report for PCT/US2008/084250, mailed Sep. 22, 2009.
Qiagen Worldwide “For rapid, high-throughput purification of plasmid DNA from high-copy vectors” DirectPrep 96 Miniprep Handbook, Nov. 2003.
Spradling et al. “Transformation of Cloned P Elements intoDrosophilaGerm Line Chromosomes”Science, vol. 218:341-347, 1982.
Sullivan et al “Chapter 18: Quantitative Microinjection ofDrosophiliaEmbryos” Cold Spring Harbor Laboratory Press, 2000, pp. 345-360.
Wieschaus, E. and Nüsslein-Volhard, C.,Drosophila: A Practical Approach, p. 200, Roberts, D.B., ed., IRL Press, Oxford, England, 1986.
Written Opinion for PCT/US2008/084250, mailed Sep. 22, 2009.

Affiliated with

Also associated with

Rating

Injecting Drosophila embryos does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Injecting Drosophila embryos, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Injecting Drosophila embryos will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4159330