Injectable culture medium for maintaining viability of myoblast

Details Injectable culture medium for maintaining viability of myoblast Injectable culture medium for maintaining viability of myoblast

1994-04-20

1996-08-06

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352403, C12N 508

Patent

active

055433162

ABSTRACT:
An injectable grade medium for maintaining cells comprising: (a) a sugar; (b) a serum albumin; (c) a calcium salt; (d) a phosphate salt; (e) a source of iron; (f) at least one salt of a Group I element; (g) at least one salt of a Group II element; (h) at least one amino acid; and (i) at least one vitamin. Components (a) through (i) are present in the medium in amounts in order to provide an osmolality of the medium of from about 320 mOsm/kg to about 550 mOsm/kg. Such an injectable grade medium has an increased osmolality as compared with conventional media, and thus such medium may be employed in order to maintain cells over extended periods of time and for injecting such cells into a desired transplantation site of a patient, whereby such cells are transplanted into the patient, and are capable of functioning in the patient.

REFERENCES:
patent: 5143842 (1992-09-01), Ham et al.
patent: 5324656 (1994-06-01), Ham et al.
patent: 5328695 (1994-07-01), Lucas et al.
Mohamed et al., In Vitro, 19(6): 471-8 (1983 Jun.).

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