Initial rate photometric method for immunoassay

Details Initial rate photometric method for immunoassay Initial rate photometric method for immunoassay

1994-09-06

1996-12-10

Spiegel, Carol A.

Chemistry: analytical and immunological testing

Involving kinetic measurement of antigen-antibody reaction

435 71, 436536, 436537, 436 34, 436171, G01N 33557

Patent

active

055830552

ABSTRACT:
An initial rate photometric immunoassay method useful for detecting and quantifying analytes in various physiological fluids is disclosed. The method is carried out by combining in a liquid medium an undiluted sample of analyte-containing physiological fluid, such as serum, and an excess of an anti-analyte antibody. A substantially constant initial rate of increase of the liquid medium's turbidity, due to the resulting immunoprecipitation reaction, is measured in real time and compared to a calibration curve prepared from known analyte concentrations to detect and quantify the analyte. Detection and quantification of numerous analytes including, haptens, drugs and proteins, in a wide variety of physiological fluids can be rapidly carried out by the present method.

REFERENCES:
patent: 4204837 (1980-05-01), Sternberg et al.
patent: 4205954 (1980-06-01), Barson
patent: 4224304 (1990-09-01), Sawai et al.
patent: 4305665 (1981-12-01), Achter et al.
patent: 4313929 (1982-02-01), Morita et al.
patent: 4446239 (1984-05-01), Tsuji et al.
patent: 4521521 (1985-06-01), Abbott et al.
patent: 4554257 (1985-11-01), Aladjem et al.
patent: 4604365 (1986-08-01), O'Neill et al.
patent: 4647544 (1987-03-01), Nicoli et al.
patent: 4766083 (1988-08-01), Miyashita et al.
patent: 4835110 (1989-05-01), Seymour et al.
patent: 5162236 (1992-11-01), Pang et al.
patent: 5169757 (1992-12-01), Yamazaki et al.
Foster, R. C. et al., "Turbiometry", Chapter 5 of Manual of Clinical Laboratory Immunology, 3d edition (1986) pp. 25-32.
Sternberg, J. C., "Rate Nephelometry", Chapter 6 of Manual of Clinical Laboratory Immunology, 3d edition (1986) pp. 33-37.
Sternberg, J. C., "A Rate Nephelometer For Measuring Specific Proteins By Immunoprecipitin Reactions", Clin Chem, 23/8, 1456-1464 (1977).
Finley, P. R., et al., "Rate-Nephelometric Inhibition Immunoassay Of Phenytoin And Phenobarbital", Clin. Chem. 27/3, 405-409 (1981).
Killingsworth et al, "Nephllometric Studies of the Precipitin Reaction: A Model System for Specific Protein Measurements," Clin. Chem. 19 (4):403-407, (1973).
Muratsugu et al, "Detection of Antistreplolysin O Antibody: Application of an Initial Rate Method of Latex Prezoelectric Immunoassay, " Anal. Chem. 64(21):2483-2487, (1992).
Savory et al, "Kinetics of the IgG-anti-IgG Reaction, as Evaluated by Conventional and Stopped-Flore Nephelometry," Clin. Chem. 20(8):1071-1075, (1974).

Affiliated with

Also associated with

Rating

Initial rate photometric method for immunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Initial rate photometric method for immunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Initial rate photometric method for immunoassay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-423732