Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1990-02-21
1993-06-29
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 721, 435 74, C12Q 170
Patent
active
052233910
ABSTRACT:
An immunoassay for identifying an inhibitor of HSV DNA replication including providing a DNA polymerizing complex that includes two complex members, the complex members being HSV DNA polymerase and UL42, providing a potential inhibitor that inhibits the binding of HSV DNA polymerase to UL42, mixing the complex members in the presence of the potential inhibitor, and determining whether the potential inhibitor inhibits formation of the complex.
REFERENCES:
Gallo et al., "Purification of the Herpes Simplex Virus Type 1 65-Kilodalton DNA-Binding Protein: Properties of the Protein, etc.", J. Virol. 62:8 (1988).
Quinn et al., "DNA Sequence of the Region in the Genome of Herpes Simplex Virus Type 1 Containing the Genes for DNA Polymerase and the Major DNA Binding Protein", Nucleic Acids Res. 13:8143 (1985).
Powell et al., "Nonstructural Proteins of Herpes Simplex Virus/Purification of the Induced DNA Polymerase", J. Virol. 24:618 (1977).
Gallo et al., "The Essential 65-Kilodalton DNA-Binding Protein of Herpes Simplex Virus Stimulates the Virus Encoded DNA Polymerase", J. Virol. 63:5023 (1989).
Meek et al., "Inhibition of HIV-1 Protease in Infected T-Lymphocytes by Synthetic Peptide Analogues", Nature 343:90 (1990).
Laemmli, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T-4", Nature 227:680 (1970).
Olivo et al., "Herpes Simplex Virus Type 1 Gene Products Required for DNA Replication: Identification and Overexpression", J. of Virol. 63:196 (1989).
McKnight et al., "Transcriptional Control Signals of a Eukaryotic Protein-Coding Gene", Science 217:316 (1982).
Taylor et al., "The Rapid Generation of Oligonucleotide-Directed Mutations at High Frequency Using Phosphorothioate-Modified DNA", Nucleic Acids Res. 13:8765 (1985).
Melton et al., "Efficient in Vitro Synthesis of Biologically Active RNA and RNA Hybridization Probes from Plasmids Containing a Bacteriophage SP6 Promoter", Nucleic Acids Res. 12:7035 (1984).
Krieg et al., "Functional Messenger RNAs are Produced by SP6 in Vitro Transcription of Cloned cDNAs", Nucleic Acids Research 12:7057 (1984).
Green et al., "Autonomous Functional Domains of Chemically Synthesized Human Immunodeficiency Virus TAT Trans-Activator Protein", Cell 55:1179 (1988).
Frankel et al., "Cellular Uptake of the TAT Protein from Human Immunodeficiency Virus", Cell 55:1189 (1988).
McGeoch et al., "The Complete DNA Sequence of the Long Unique Region in the Genome of Herpes Simplex Virus Type 1", J. Gen. Virol. 69:1531 (1988).
Fechheimer et al., "Transfection of Mammalian Cells with Plasmid DNA by Scrape Loading and Sonication Loading", Proc. Nat. Aca. Sci. 84:8463 (1987).
Dreyer et al., "Inhibition of Human Immunodeficiency Virus 1 Protease in Vitro: Rational Design of Substrate Analogue Inhibitors", Proc. Nat. Aca. Sci. 86:9752 (1989).
Yager et al., "Analysis of the Transcript of the Herpes Simplex Virus DNA Polymerase Gene Provides Evidence", etc., J. Virol 62:2007 (1988).
Elias et al., "A DNA Binding Protein Specific for an Origin of Replication of Herpes Simplex Virus Type 1", Proc. Nat. Aca. Sci. 83:6322 (1986).
Wu et al., "Identification of Herpes Simplex Virus Type 1 Genes Required for Origin-Dependent DNA Synthesis", J. Virol. 62:435 (1988).
Lobel et al., "Construction of Mutants of Moloney Murine Leukemia Virus by Suppressor-Linker Insertional Mutagensis: Positions of Viable Insertion Mutations", Proc. Nat. Aca. Sci. 81:4149 (1984).
De Luca et al., "Isolation and Characterization of Deletion Mutants of Herpes Simplex Virus Type 1 in the Gene, etc.", J. of Virol. 56:558 (1985).
Gibbs et al., "Sequence and Mapping Analyses of the Herpes Simplex Virus DNA Polymerase Gene Predict A C-Terminal Substrate Binding Domain", Proc. Natl. Acad. Sci USA 82:7969 (1985).
Dutia et al., "Specific Inhibition of Herpesvirus Ribonucleotide Reductase by Synthetic Peptides", Nature 321:439 (1986).
This invention was made with Government support, and the U.S. Government therefore has certain rights in the invention.
Coen Donald M.
Digard Paul E.
President and Fellows of Harvard College
Saunders David
Wortman Donna C.
LandOfFree
Inhibitors of herpes simplex virus replication does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Inhibitors of herpes simplex virus replication, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inhibitors of herpes simplex virus replication will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1754545