Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase

Details Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase

1983-01-05

1985-08-13

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351721, 435 91, 435253, 435317, 435232, 536 27, 935 14, 935 29, 935 72, C12N 1500, C12N 120, C12N 100, C12P 1934

Patent

active

045350604

ABSTRACT:
Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3-phosphoshikimate synthetase (EC: 2.5.1.19) (ES-3-P synthetase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme.
The E. coli strain C600(pPMG1) has been deposited at the A.T.C.C. on Dec. 14, 1982 and been given A.T.C.C. accession no. 39256.

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