Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase,

Details Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase,

1988-08-30

1992-03-10

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435193, 536 27, 935 60, 935 79, C12N 910, C12N 1554, C12N 1500

Patent

active

050949454

ABSTRACT:
Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3-phosphoshikimate synthase (EC: 2.5.1.19) (ES-3-P synthase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme.
The E. coli strain C600 (pPMG1) has been deposited at the A.T.C.C. on December 14, 1982 and given A.T.C.C. Accession No. 39256.

REFERENCES:
patent: 4535060 (1985-08-01), Comai
patent: 4769061 (1988-09-01), Comai
patent: 4971908 (1990-11-01), Kishore et al.
LeVine et al., Journal of Bacteriology, 1980, vol. 143, #2, pp. 1081-1085.

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