Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase,

Details Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase,

1985-02-04

1988-09-06

Wiseman, Thomas G.

Chemistry: fertilizers

Processes and products

Forms or conditioning

71 91, 71 92, 71 93, 71107, 71108, 71109, 71110, 71115, 71116, 71117, 71118, 800 1, 4351723, 435193, 4352404, 935 14, 935 67, 935 64, A01N 5700, A01N 4302, A01N 4318, A01N 4364, A01N 3710, A01N 3736, A01N 3738, A01N 3710, A01N 3738, A01N 3718, C12N 500, C12N 1500, C12N 910

Patent

active

047690615

ABSTRACT:
Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3- phosphoshikimate synthase (EC: 2.5.1.19) (ES-3-P synthase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme.
The E. coli strain C600(pPMG1) has been deposited at the A.T.C.C. on Dec. 14, 1982 and given A.T.C.C. Accession No. 39256.

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