Inhibition of vascular smooth muscle cell proliferation with imp

Details Inhibition of vascular smooth muscle cell proliferation with imp Inhibition of vascular smooth muscle cell proliferation with imp

1995-06-02

1998-06-16

Naff, David M.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Animal or plant cell

424425, 424426, 435174, 435177, 435180, 435395, 435397, 435398, 435402, C12N 1100, C12N 1108, C12N 500

Patent

active

057665842

ABSTRACT:
A composition and method are provided for inhibition of vascular smooth muscle cell proliferation following injury to the endothelial cell lining of a blood vessel such as resulting from angioplasty, vascular bypass surgery or organ transplantation. The composition is a matrix such as a biodegradable hydrogel made of a synthetic polymer, protein or polysaccharide seeded with vascular endothelial cells which can be xenografts, allografts or autografts, or genetically engineered cells. Attachment of cells to the matrix can be enhanced by coating with collagen, laminin, fibronectin, fibrin, basement membrane components or attachment peptides. Biologically active compounds such as anti-inflammatory agents may also be contained in the matrix. In the method, the matrix containing endothelial cells is implanted in a patient at a site adjacent the injury such as by wrapping the matrix around the blood vessel. The endothelial cells secrete products that diffuse into surrounding tissue but do not migrate to the endothelial cell lining of the blood vessel. The endothelial cells may be obtained by biopsy of the patient into which the matrix is implanted and the cells can be cultured in the matrix in vitro and then implanted in vivo.

REFERENCES:
patent: 4418691 (1983-12-01), Yannas et al.
patent: 4787900 (1988-11-01), Yannas
patent: 5399665 (1995-03-01), Barrera et al.
patent: 5455039 (1995-10-01), Edelman et al.
patent: 5527532 (1996-06-01), Edelman et al.
patent: 5540928 (1996-07-01), Edelman et al.
patent: 5567612 (1996-10-01), Vacanti et al.
Bjornsson et al., "Acidic Fibroblast Growth Factor Promotes Vascular Repair," Proc. Natl. Acad. Sci. USA, 88: 8651-8655 (1991).
Castellot et al., "Cultured Endothelial Cells Produce a Heparinlike Inhibitor of Smooth Muscle Cell Growth," J. Cell Biol., 90: 372-9 (1981).
Cooke et al., "Cellular Mechanisms of Atherogenesis and the Effects of Nitric Oxide," Curr. Opin. Cardiol., 7:799-804 (1992).
Edelman, E.R. & Karnovskv, M.J. "Contrasting Effects of the Intermittent and Continuous Administration of heparin in Experimental Restenosis," Circ. 89: 770-776 (1994).
Edelman, et al., "Basic Fibroblast Growth Factor Enhances the Coupling of Intimal Hyperplasia and Proliferation of Vasa Vasorum in Injured Rat Arteries," J. Clin. Invest. 89:465-471 (1992).
Ellis et al., "Effect of 18-to 24-hour Heparin Administration for Prevention of Restenosis After Uncomplicated Coronary Angioplasty," Am. Heart. J., 117: 777-782 (1989).
Farndale, et al., "Improved Quantitation and Discrimination of Sulphated Glycosaminoglycans by Use of Dimethylmethylene Blue," Biochimica et Biophysica Acta 883:173-177 (1986).
Fishman et al., "Endothelial Regeneration in the Rat Carotid Artery and the Significance of Endothelial Denudation in the Pathogenesis of Myointimal Thickening," Lab. Invest., 32: 339-51 (1975).
Gimbrone, M., "Culture of Vascular Enendothelium," Progress Hemostasis and Thrombosis 3:1-28 (1976).
McNamara, et al., "L-Arginine Inhibits Balloon Catheter-Induced Intimal Hyperplasia," Biochem. Biophys. Res. Comm., 193: 291-296 (1993).
Moncada et al., "The L-Arginine-Nitric Oxide Pathway," N. Engl. J. Med., 329: 2002-2012 (1993).
Nugent et al., "Vascular Cell-Derived Heparan Sulfate Shows Coupled Inhibition of Basic Fibroblast Growth Factor Binding and Mitogenesis in Vascular Smooht Muscle Cells," Circulation Research, 73: 1051-1060 (1993).
Schwartz et al., "The Aortic Intima. II. Repair of the Aortic Lining After Mechanical Denudation," Am. J. Pathol., 81: 15-42 (1975).

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