Inhibition of protein kinase activity by aptameric action of oli

Details Inhibition of protein kinase activity by aptameric action of oli Inhibition of protein kinase activity by aptameric action of oli

1995-04-04

1999-12-07

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, 536 243, 935 76, 935 77, 935 78, C07H 1900, C07H 2102, C07H 2104, C12N 1500

Patent

active

059985968

ABSTRACT:
The present invention are oligonucleotides that specifically bind to and directly inhibit the biological function of target molecules such as proteins, peptides or derivatives. The direct or aptameric interaction of oligonucleotides of the present invention with proteins, peptides and derivatives represents a non-antisense mediated effect. The oligonucleotides have been shown to bind to isolated target molecules and to inhibit biological function of the target molecule within cells. In particular, the oligonucleotides have been shown to directly inhibit the kinase activity of protein-tyrosine kinase. The oligonucleotides of the present invention have significant beneficial effects against a chronic myelogenous leukemia derived cell line as demonstrated using cellular phosphotyrosine content as well as cellular growth in soft agar.

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