Inhibition of protein degradation in living cells with dipeptide

Details Inhibition of protein degradation in living cells with dipeptide Inhibition of protein degradation in living cells with dipeptide

1994-04-20

1998-06-16

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435 701, 435 711, 435244, 435325, 435363, 435366, 435377, 514 2, C12N 116, C12N 138, A61K 3805, C12P 2100

Patent

active

057669279

ABSTRACT:
The half-life of a Type I, II and III non-compartmentalized intracellular proteins is increased in living eukaryotic cells by contacting the cells with a regulator having an amino-terminal amino acid residue which is the same or similar to the amino-terminal residue of the intracellular protein. The regulator is a dipeptide, a small polypeptide or a carboxyl-terminal derivative of an amino acid. The dipeptide or small polypeptide has an N-terminal amino acid residue which is Arg, Lys or His for the Type I protein, Phe, Leu, Trp, Tyr or Ile for the Type II protein and Ala, Ser or Thr for the Type III protein. The carboxyl-terminal derivative of an amino acid may be an amino acid modified at its C-terminus by the addition of a group selected from methyl, ethyl, propyl, butyl and isobutyl. The amino acid modified is the N-terminal amino acid residue of the dipeptide or small polypeptide for the respective Type I, II and III proteins. Compositions may be formed containing the regulator for contacting with the cells. Increasing the half-life of intracellular protein with the regulator may be used for treating diseases resulting from an abnormal breakdown of a desired protein, and for enhancing in vivo production of a desired protein.

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