Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-03-10
1999-01-26
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 792, 435 794, 435962, 435967, 435 7021, 43524027, 435 22, 435 23, 435 24, 435183, 435184, 435219, G01N 33567
Patent
active
058637427
ABSTRACT:
A method of inhibiting proteolytic degradation of a thermally-stable intracellular protein is described. The method involves adding 1 or more denaturing agents to a sample which contains the protease and the protein of interest and heating the resulting solution at a temperature and for period of time sufficient to denature the protease. The method optionally includes a step for lysing the cell if the protein of interest is contained in a cell in order to release said protein. Additionally, a method of determining Mx protein induced by interferon in a blood sample is described. The method involves adding to a blood sample a lysing agent, a denaturing agent, and a detergent selected to solubilize Mx protein. The sample containing Mx protein is then heated at a temperature of from about 50.degree. C. to about 60.degree. C. for a period of time of from about 1 minute to about 30 minutes, and the Mx protein in the solution then is determined. Also described is a solution including a synthetic matrix mimicking whole blood cell lysates, or actual whole blood cell lysates, to which a known concentration of a thermally-stable intracellular protein is added to prepare a control material. In addition, a solution free of a protease that degrades the intracellular protein is disclosed, such solution remaining stable at 4.degree. C. for at least three weeks.
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Oh Se-Kyung
Towbin Harry
Blackburn Robert P.
Chiron Diagnostics Corporation
Hutzell Paula K.
Morgenstern Arthur S.
Prickril Benet
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