Inhibition of proliferation of vascular smooth muscle cell

Details Inhibition of proliferation of vascular smooth muscle cell Inhibition of proliferation of vascular smooth muscle cell

1995-02-16

1999-02-09

Rories, Charles C.P.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

435375, 435377, 536 245, 536 243, 536 2431, 536 2433, 424450, A61K 4800, C12N 510, C07N 2100

Patent

active

058694625

ABSTRACT:
This invention encompasses a method for inhibiting vascular cellular activity of cells associated with vascular lesion formation in mammals which involves administering an effective dosage of at least one antisense sequence to at least one gene expressing a cyclin or a cyclin dependent kinase which inhibits the expression of the gene. More particularly, the invention involves administering antisense sequences which inhibit the expression of cyclin A, B1, B2, C, D1, D2, D3, E or cyclin X (p46) cyclin X and cyclin dependent kinase cdc2, cdk2, cdk4 or cdk5. It is preferable to use two antisense sequences each from a different cyclin or cyclin dependent kinase. The cyclin or cyclin kinase depending kinase dosage is preferable administered in combination with proliferating cell nuclear antigen (PCNA). Antisense methods and compositions direct to inhibiting the expression of growth factors such as TGF-.beta..sub.1, TGF, bFGF, PDGF are also contemplated by the present invention.
The antisense sequences are incorporated into liposomes, particularly liposomes containing HVJ and which are directly administered intraluminally, intramurally or periadventitiously.
The methods of this invention are useful in treating a broad spectrum of vascular lesions such as lesions in the carotid femoral and renal arteries, particularly lesions resulting from renal dialysis fistulas. The invention is particularly useful in treating vascular grafts ex-vivo and prior to surgical grafting.

REFERENCES:
patent: 5585479 (1996-12-01), Hoke et al.
patent: 5593974 (1997-01-01), Rosenberg et al.
N. Milner et al. Nature Biotechnology 15:537-41, Jun. 15, 1997.
P. Felguer et al. PNAS 84:7413-7 ('87).
J. Wang et al. Nature 343:555-7 ('90).
J. Pines et al. Cell 58:833-46 ('89).
D. Walker et al. Nature 354:314-7 ('91).
J. Doyle et al. Nucl. Ac. Res. 18(16) 4751-7 ('90).
J. Holz et al. Mol. Cell. Biol. 8(2):963-73 ('88).
R. Mosley et al., Am. J. Gastroent. 89(10) 1874-9 ('94) (abstract).
D. Liu et al. Clin. Exp. Hypertrans. 16(4) 391-414 ('94) (abst.).
P. Libby et al. Circulation 86(suppl. III) 47-52 ('92).
M. Mann et al. PNAS 92:4502-6 ('95).
E. Uhlmann et al. Chem. Rev. 90(4):543-84 ('90).
J. Milligan et al. J. Med. Chem. 31(14)1923-37 ('93).
C. Stein et al. Science 261:1004-12 ('93).
B. Tseng et al. Cancer Gene Ther. 1(1)65-71 ('94).
P. Wessermann et al. Biomed Biochim Acta 48(1)85-93 ('89).
R. Stull et al. Pharmacol. Res. 12(4) 465-83 ('95).
W. James Annivir. Chem. & Chemotherap. 2(4) 191-214 ('91).
I. Solodin et al. Biochem. 34:13537-44 ('95).
D. Juskulski et al. Science 240:1544-6 ('88).
Y. Furukawa et al. Science 250:805-8 ('90).
Y. Kaneda et al. Exp. Cell Res. 173:56-59 ('87).
O'Connor, P.M., et al. G.sub.2 Delay Induced by Nitrogen Mustard in Human Cells Affects Cyclin A/cdk2 and Cyclin B1/cdc2-Kinase Complexes Differently, J. Biol. Chem., vol. 268, No. 11, pp. 8298-8308, Apr. 15, 1993.
Kato, Keiko, et al. "Expression of Hepatitis B Virus Surface Antigen in Adult Rat Liver" J. Biol. Chem., vol. 266, No. 6, pp. 3361-64, Feb. 25, 1991.
Rekhter, M. et al. "Cell Proliferation in Human Arteriovenous Fistulas Used For Hemodialysis" Arteriosclerosis and Thrombosis, vol. 13, No. 4 pp. 609-617 Apr., 1993.
Williams, Richart T. et al. "Identification of a Novel Cyclin-like Protein in Human Tumor Cells" J. Biol. Chem., vol. 268, No. 12, pp. 8871-8880, Apr. 25, 19.
Ajchenbaum, Florence, et al. "Independent Regulation of Human D-type Cyclin Gene Expression during G.sub.1 Phase in Primary Human T Lymphocytes".
J. Biol. Chem., vol. 268, No. 6, pp. 4113-4119, Feb. 25, 1993.
Dzau, V. (1986) Hypertension 8:553-559.
Simons, M., et al. (1992) Nature 359:67-70.
Simons, M. and Rosenberg, R. (1992) Circulation Research 70:835-843.
Speir, E. and Epstein, S. (1992) Circulation 86:538-547.
Rainer, et al. (1991) Hypertension 18:326, abstract 47.
Itoh, et al. (1991) Hypertension 18:325, abstract 46.
Dzau, V. (1988) Circulation 77 (suppl I) 14-113.
Itoh, et al. (1990) J. Clin. Invest. 86:1690-1697.
Campbell-Boswell and Robertson (1981) Exp. and Mol. Pathology 35:265-276.
Naftilan, et al. (1989) Hypertension 13:706-711.
Itoh, et al. (1991) Biochem. Biophys. Res. Comm. 176:1601-1609.
Geisterfer, et al. (1988) Circulation Research 62:749-756.
Gibbons, et al. (1990) Clin. Research 38:287A.
Ferns, et al. (1991) Science 253:1129-1132.
Daemen, et al. (1991) Circulation Research 68:450-456.
Linder, et al. (1991) Circulation Research 68:106-113.
Majesky, et al. (1991) J. Clin. Invest. 88:914-910.
Sarzani, et al. (1989) J. Clin. Invest. 83:1404-1408.
Naftilan, et al. (1989) J. Clin. Invest. 83:1419-1424.
Powell, et al. (1989) Science 245:186-188.
Barret and Benditt (1988) P.N.A.S. 85:2810-2814.

Affiliated with

Also associated with

Rating

Inhibition of proliferation of vascular smooth muscle cell does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Inhibition of proliferation of vascular smooth muscle cell, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inhibition of proliferation of vascular smooth muscle cell will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1948506