Inhibition of phospholipase A.sub.2 to reduce neuronal cell deat

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 6, 435325, 435375, 435377, C12Q 100, C12Q 168, C12N 506

Patent

active

058663185

ABSTRACT:
The invention is drawn to a method for identifying agents that inhibit neural degeneration by administering to cell populations consisting essentially of neurons or cells from neuronal cell lines, where these cells are exposed to an apoptotic stimulus other than APP gene products, an agent, where it is determined whether the agent inhibits neural degeneration.

REFERENCES:
patent: 5137873 (1992-08-01), Yankner
patent: 5276059 (1994-01-01), Caughey et al.
patent: 5281626 (1994-01-01), Oinuma et al.
patent: 5328842 (1994-07-01), Chiou et al.
patent: 5354677 (1994-10-01), Knopf et al.
patent: 5453443 (1995-09-01), Perrier et al.
patent: 5478857 (1995-12-01), Clemens et al.
Agarwal et al "Phospholipase Activation Triggers Apoptosis is Photosenstized Mouse lymphoma Cells"Cancer Research vol. 53:5897-5902, Dec. 15, 1993.
Lockhart et al. "Inhibitors of Free Radical Formation Fail to Attenuate Direct beta-Amyloid25-35 Peptide-Mediated Neurotoxicity in Rat Hippocampal Cultures", Journal of Neuroscience Research, vol. 39, No. 4, pp. 495-505, 1994.
Sipe "Amyloidosis", Annual Review of Biochemistry, vol. 61, pp. 947-975, 1992.
Eikelenboom, Piet et al., Inflammatory mechanisms in Alzheimer's disease, Tips, vol. 15, Dec. 1994.
Simmons, Linda K., et al., "Secondary Structure of Amyloid .beta. Peptide Correlates with Neurotoxic Activity in Virto," The American Society for Pharmacology and Experimental Therapeutics, Molecular Pharmacology, 45:373-379, Mar. 1994.
Dumuis, Aline, et al., Stimulation by Glutamate Receptors of Arachidonic Acid Release Depends on the Na.sup.+/ Ca.sup.2+ Exchanger in Neuronal Cells, The American Society for Pharmacology and Experimental Therapeutics, Molecular Pharmacology, 43:976-981, Jun. 1993.
Alberts, Bruce, et al., Molecular Biology of The Cell, Second Edition, 1989.
Abdullah et al., "Human cytosolic phospholipase A.sub.2 expressed in insect cells is extensively phosphorylated on Ser-505," Biochimica et Biophysica Acta, 1244:157-164 (1995).
Abdullah et al., "Synthesis and Preparation of an Affinity Chromatography Column for the Purification of Cytosolic Phospholipase A.sub.2," Bioorganic & Medicinal Chemistry Letters,5(5):519-522 (1995).
Abdullah et al., "Purification of Baculovirus-Overexpressed Cytosolic Phospholipase A.sup.2 Using a Single-Step Affinity Column Chromatography," Protein Expression and Purification, 6:291-297 (1995).
Ackermann et al., "Inhibition of Macrophage Ca.sup.2+ -independent Phospholipase A.sub.2 by Bromoenol Lactone and Trifluoromethyl Ketones," J. Biol. Chem., 270 (1):445-450 (1995).
Bartoli et al., "Tight Binding Inhibitors of 85-kDa Phospholipase A.sub.2 but Not 14-kDa Phospholipase A.sub.2 Inhibit Release of Free Arachidonate in Thrombin-stimulated Human Platelets," J. Biol. Chem., 269(22):15625-15630 (1994).
Currie et al., "Phosphorylation and activation of Ca.sup.2+ -sensitive cytosolic phospholipase A.sub.2 MCII mast cells mediated by high-affinity F.sub.c receptor for IgE," Biochem. J., 304:1-6 (1994).
Dennis, "Diversity of Group Types, Regulation, and Function of Phospholipase A.sub.2," J. Biol. Chem., 269(18):10357-13060 (1994).
Glaser et al., "Phospholipase A.sub.2 enzymes: regulation and inhibition," TiPS, 14:92-98 (1993).
Kramer, "Structure, Function and Regulation of Mammalian Phospholipases A.sub.2 ,"Advances in Second Messenger and Phosphoprotein Research, 28:81-89 (1993).
Lin et al., "cPLA.sub.2 Is Phosphorylated and Activated by MAP Kinase," Cell, 72:269-278 (1993).
Mattson et al., ".beta.-Amyloid precursor protein metabolites and loss of neuronal Ca.sup.2+ homeostasis in Alzheimer's disease," TINS, 16(10):409-414 (1993).
Meda et al., "Activation of microglial cells by .beta.-amyloid protein and interferon-.tau.," Nature, 374:647-650 (1995).
Oinuma et al., "Synthesis and Biological Evaluation of Substituted Benzenesulfonamides as Novel Potent Membrane-Bound Phospholipase A.sub.2 Inhibitors," J. Med. Chem., 34:;2260-2267 (1991).
Petit et al., "Isolation and Characterization of a Cytosolic Phospholipase A.sub.2 from Bovine Adrenal Medulla," J. of Neurochem. 64:139-146 (1995).
Reynolds et al., "1-Hexadecyl-2-Archidonoylthio-2-deoxy-sn-Glycero-3-Phosphorylcholine as a Substrate for Microtiterplate Assay of Human Cytosolic Phospholipase A.sub.2," Anal. Biochem., 217:25-32 (1994).
Reynolds et al, "Assay Strategies and Methods for Phospholipases," Methods in Enzymology, 197:3-23 1991).
Riendeau et al., "Arachidonyl Trifluoromethyl Ketone, a Potent Inhibitor of 85-kDa Phospholipase A.sub.2, Blocks Production of Arachidonate and 12-Hydroxyeicosa-tetraenoic Acid by Calcium Ionophore-challenged Platelets," J. Biol. Chem., 269(22):15619-15624 (1994).
Roshak et al., "Suppression of Monocyte 85-kDa Phospholipase A.sub.2 by Antisense and Effects on Endotoxin-induced Prostaglandin Biosynthesis," J. Biol. Chem., 269(42):25999-26005 (1994).
Street et al., "Slow-and Tight-Binding Inhibitors of the 85-kDa Human Phospholipase A.sub.2," Biochem., 32:5935-5940 (1993).
Verity, "Mechanisms of Phospholipase A.sub.2 Activation and Neuronal Injury," Annals NY Acad. Sci., 379:110-120 (1993).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Inhibition of phospholipase A.sub.2 to reduce neuronal cell deat does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Inhibition of phospholipase A.sub.2 to reduce neuronal cell deat, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inhibition of phospholipase A.sub.2 to reduce neuronal cell deat will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1115970

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.