Inhibition of interfering endogenous enzyme activity in assays o

Details Inhibition of interfering endogenous enzyme activity in assays o Inhibition of interfering endogenous enzyme activity in assays o

1993-07-16

1997-03-11

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 23, 435 19, 435 28, 435 791, 435 14, 436501, C12Q 137, C12Q 126, C12Q 151, G01N 33566

Patent

active

056100256

ABSTRACT:
The invention describes biological assays in which hydrogen peroxide is used as an oxidizing agent, or wherein hydrogen peroxide is used to oxidize a dye or other intermediate to generate a detectable species. The stability of the hydrogen peroxide in the presence of at least one other enzyme which decomposes hydrogen peroxide is enhanced by the addition of a suitable inhibitor for the enzyme and the inhibitor does not substantially inhibit enzymes used in the assay. When catalase is the enzyme to be inhibited, catalase inhibitors that can be used in the biological systems include hydroxylamine sulfate. The enzyme inhibitor can be incorporated in an integral analytic device.

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Barrier, W. R., vol. 45/11-B of Dissertation Abstracts International, p. 3382, 1984. (Abstract).

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