Inhibition of cell migration with synthetic peptides

Details Inhibition of cell migration with synthetic peptides Inhibition of cell migration with synthetic peptides

1993-12-17

1996-08-20

Low, Christopher S. F.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

514 18, 514 17, 530324, 530300, 4352402, 435 723, A61K 3804, A61K 3807, A61K 3816

Patent

active

055479367

ABSTRACT:
A method of inhibiting the invasion of cells, particularly malignant cells through an extracellular membrane by contacting the membrane-cell interface with synthetic Arg-Gly-Asp-containing peptides. In one embodiment, the invention provides peptides containing the amino acid sequence Arg-Gly-Asp-Thr, more specifically Gly-Arg-Gly-Asp-Thr-Pro, which inhibits the attachment of cells to type I collagen in addition to fibronectin and vitronectin, and a method of inhibiting the attachment of cells to type I collagen. The invention further provides an assay for quantitating the invasive quality of cells by determining the amount of such peptides necessary to prevent the cells from penetrating an extracellular membrane, such as an amniotic membrane, in vitro.

REFERENCES:
patent: 4578079 (1986-03-01), Ruoslahti et al.
patent: 4614517 (1986-09-01), Ruoslahti et al.
patent: 4988621 (1991-01-01), Ruoslahti et al.
McCarthy et al. 1986. J. Cell. Biol. 102, 179-188.
Chambers Science and Technolog Dictionary (Walker et al., eds.) 1988. W. R. Chambers Ltd. and Cambridge Univ. Press. Cambridge, pp. 29, 284, 566, 863,78. (Supplied by appl. in Prelim. amendment).
Humphries et al. 1986. Science 233, 467-470.
Hendrix et al. 1985. Clin. Exp. Metastasis 3, 221-223.
Pierschbacher et al. 1984, Nature, 309, 30--33.
Goodman et al. 1985 Embo J. 4, 2769-2771.
Gehlsen et al. 1987, J. Cell Biol. 105(4 part 2) abstract 236, p. 44a.
Pierschbacher et al., Influence of Stereochemistry of the Sequence Arg-Gly-Asp-XAA on Binding Specificity in Cell Adhesion. J. Biol. Chem. 262(36):17294-17298 (1987).
Gehlsen et al., Inhibition of In Vitro Tumor Cell Invasion by Arg-Gly-Asp-Containing Synthetic Peptides. Chem. Abstracts. 108:391, Abstract No. 219712r (1988).
Gehlsen et al., Inhibition of In Vitro Tumor Cell Invasion by Arg-Gly-Asp-Containing Synthetic Peptides. J. Cell. Biol. 106(3):925-30 (1988).
Dedhar et al., "A Cell Surface Receptor Complex for Collagen Type I Recognizes the Arg-Gly-Asp Sequence" J. Cell Biol. 104:585-593 (1987).

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