Inhibition of apoptosis by the expression of antisense RNA...

Details Inhibition of apoptosis by the expression of antisense RNA... Inhibition of apoptosis by the expression of antisense RNA...

2005-09-13

2005-09-13

McGarry, Sean (Department: 1635)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S320100, C435S006120, C536S024500

Reexamination Certificate

active

06943022

ABSTRACT:
The present invention relates to an antisense nucleotide of caspase-3, an expression vector encoding antisense RNA of caspase-3, and an inhibition method of cellular apoptosis in recombinant Chinese hamster ovary (CHO) cells using the same. More precisely, the present invention relates to an antisense nucleotide composed of a base sequence represented by Sequence ID No. 1, which suppresses the expression of caspase-3, an expression vector containing the above antisense nucleotide and expressing antisense RNA of caspase-3, and an inhibition method of apoptosis of recombinant cells by suppressing the expression of caspase-3 through introducing the above expression vector into the recombinant cells and then expressing antisense RNA of caspase-3. According to the present invention, apoptosis can be inhibited by suppressing the activation of caspase-3, which is related to the apoptosis, and the integrity of target protein produced in the recombinant cells can be enhanced.

REFERENCES:
patent: 6303374 (2001-10-01), Zhang et al.
patent: 2002/0001806 (2002-01-01), Huang et al.
U. Galderisi et al., “Antisense Oligonucleotides as Therapeutic Agents,” Jounral of Cellular Physiology, 181:251-257, 1999.
S.L. McKenna et al., “Inhibition of Caspase Activity Delays Apoptosis in a Transfected NS/0 Myeloma Cell Line,” Biotechnology and Bioengineering, 67(2):165-176, Jan. 20, 2000.

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